21 Result(s)
within
Jae-Kwang Kim
Plant Sciences
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Article
Chemical profiling of insect-resistant rice shows that geographical variations produce greater differences in chemical composition than genetic modifications
Genetically modified (GM) rice (event, Bt-T) contains the Bacillus thuringiensis gene for insecticidal properties. To evaluate changes in the chemical compositions of Bt-T and non-GM rice cultivated in two region...
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The Tomato Juhwang Mutant Accumulates a High Level of β-Carotene and has a Long Shelf-Life
Carotenoids have diverse functions in plants and animals. In tomato (Solanum lycopersicum), the red color of fruits is due to the accumulation of the carotenoid lycopene during fruit ripening. Here, the effect of...
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Metabolic profiling and antioxidant properties of hybrid soybeans with different seed coat colors, obtained by crossing β-carotene-enhanced (Glycine max) and wild (Glycine soja) soybeans
We obtained new hybrid soybeans (Hybrid) with different seed coat colors such as green (Hybrid-GR), brown (Hybrid-BR), yellow (Hybrid-YE), and black (Hybrid-BL), by hybridizing β-carotene-enhanced soybeans (BCE;
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Decontamination of DNA in Taq DNA polymerase reagents using nylon membranes for monitoring of GMOs
Polymerase chain reaction (PCR) is one of the most commonly used methods in diagnostic and molecular biology. However, DNA contamination can lead to incorrect results. This poses a major problem, especially in...
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Article
Reciprocal Crosses Between Astaxanthin and Capsanthin Rice Unravel Effects of Metabolic Gene Efficacy in Rice Endosperm
Carotenoids are not naturally accumulated in the endosperms of rice, which is a cereal crop. In our previous study, biofortified rice seeds accumulated two red color carotenoids, ketocarotenoids including asta...
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Article
Natural hybridization between transgenic and wild soybean genotypes
Cultivation of transgenic soybean lines [Glycine max (L.) Merr.] and the international trade of their seeds has led to an increased focus on the potential risk of gene flow from transgenic to wild soybean (Glycin...
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Article
Metabolic profiling and antioxidant activity during flower development in Agastache rugosa
Our previous study showed that flowers of Agastache rugosa had higher phenolic levels and higher antibacterial and antioxidant capacity compared to those of the leaves and stems. The aim of this study was to prov...
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Article
Alteration of Carotenoid Metabolic Machinery by β-Carotene Biofortification in Rice Grains
To increase nutritional values as dietary sources of provitamin A and health-promoting antioxidants in rice grains, carotenoids have been biofortified in a Golden Ricelike variety, the stPAC (stPsy-2A-stCrtI) ric...
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Article
Open AccessHigh accumulation of γ-linolenic acid and Stearidonic acid in transgenic Perilla (Perilla frutescens var. frutescens) seeds
Polyunsaturated fatty acids such as linoleic acid (LA) and α-linolenic acid (ALA) are abundant in vegetable oils and are important for human health. In the body, LA and ALA are respectively converted to the om...
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Article
Effect of codon optimization on the enhancement of the β-carotene contents in rice endosperm
A β-carotene is the most well-known dietary source as provitamin A carotenoids. Among β-carotene-producing Golden Rice varieties, PAC (Psy:2A:CrtI) rice has been previously developed using a bicistronic recombina...
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Article
In planta cleavage of carotenoids by Arabidopsis carotenoid cleavage dioxygenase 4 in transgenic rice plants
A family of carotenoid cleavage dioxygenases (CCDs) produces diverse apocarotenoid compounds via the oxidative cleavage of carotenoids as substrates. Their types are highly dependent on the action of the CCD f...
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Article
Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1
RsMYB1, a MYB TF of red radish origin, was characterized as a positive regulator to transcriptionally activate the anthocyanin biosynthetic machinery by itself in ...
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Article
Monthly metabolic changes and PLS prediction of carotenoid content of citrus fruit by combined Fourier transform infrared spectroscopy and quantitative HPLC analysis
This work examined the potential of Fourier transform infrared spectroscopy (FT-IR) spectroscopy and high-performance liquid chromatography (HPLC) analysis in evaluating metabolic changes during ripening of ci...
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Article
Expression of tobacco tocopherol cyclase in rice regulates antioxidative defense and drought tolerance
Tocopherols (α-, β-, γ-, and δ-tocopherol) represent a group of lipophilic antioxidants that are synthesized only by photosynthetic organisms. It is widely believed that the main functions of tocopherols are p...
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Article
N-terminal region of rice polycomb group protein OsEZ1 is required for OsEZ1–OsFIE2 protein interaction
Polycomb group (PcG) proteins form Polycomb Repressive Complex 2 (PRC2), which regulates seed development by the epigenetic control of gene expression. Interaction assay among Arabidopsis Fertilization-independen...
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Article
Petal-specific activity of the promoter of an anthocyanidin synthase gene of tobacco (Nicotiana tabacum L.)
Anthocyanidin synthase (ANS) is a key enzyme in the late stages of the biosynthesis of anthocyanins, an important class of plant pigments. Two ANS genes (NtANS1 and NtANS2) were cloned from flowers of the tobacco...
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Article
Unintended polar metabolite profiling of carotenoid-biofortified transgenic rice reveals substantial equivalence to its non-transgenic counterpart
Substantial equivalence is a critical concept for biosafety assessment of genetically modified (GM) crops. To investigate substantial equivalence among carotenoid-biofortified GM rice and five conventional ric...
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Use of an anthocyanin production phenotype as a visible selection marker system in transgenic tobacco plant
To develop a potentially alternative method for the selection of transgenic plants instead of antibiotic and herbicide resistance, anthocyanin pigmentation phenotype was examined to provide a visible selection...
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Article
Compositional comparative analysis between insect-resistant rice (Oryza sativa L.) with a synthetic cry1Ac gene and its non-transgenic counterpart
Composition analysis of genetically modified crops is an important consideration in the assessment of food safety. Agb0101 (Oryza sativa L. cv. Nakdongbyeo) developed in Korea is a form of insect-resistant rice t...
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Article
Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter
Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here...
