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Article
Early life changes in histone landscape protect against age-associated amyloid toxicities through HSF-1-dependent regulation of lipid metabolism
Transient events during development can exert long-lasting effects on organismal lifespan. Here we demonstrate that exposure of Caenorhabditis elegans to reactive oxygen species during development protects agains...
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Article
Characterization of the cellular lipid composition during SARS-CoV-2 infection
Emerging and re-emerging zoonotic viral diseases continue to significantly impact public health. Of particular interest are enveloped viruses (e.g., SARS-CoV-2, the causative pathogen of COVID-19), which inclu...
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Article
High concentrations of urinary ethanol metabolites in neonatal intensive care unit infants
Infants in the neonatal intensive care unit may be exposed to ethanol via medications that contain ethanol as an excipient and through inhalation of ethanol vapor from hand sanitizers. We hypothesized that bot...
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Article
Development and bioanalytical method validation of an LC-MS/MS assay for simultaneous quantitation of 2-alkyl-4(1H)-quinolones for application in bacterial cell culture and lung tissue
Pseudomonas aeruginosa is an opportunistic pathogen that produces numerous exoproducts during infection that help it evade the host immune system and procure nutrients from the host environment. Among these produ...
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Article
Alternatively Activated Macrophages Are the Primary Retinoic Acid-Producing Cells in Human Decidua
In situ production and metabolism of all-trans retinoic acid (RA) in decidual tissue are critically important for endometrial stromal differentiation, embryo implantation, and healthy placentation. However, th...
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Article
Open AccesscPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury
The autophagy–lysosomal pathway plays an essential role in cellular homeostasis as well as a protective function against a variety of diseases including neurodegeneration. Conversely, inhibition of autophagy, ...
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Article
Open AccessNoncoding dsRNA induces retinoic acid synthesis to stimulate hair follicle regeneration via TLR3
How developmental programs reactivate in regeneration is a fundamental question in biology. We addressed this question through the study of Wound Induced Hair follicle Neogenesis (WIHN), an adult organogenesis...
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Article
Targeted Metabolomics Identifies Pharmacodynamic Biomarkers for BIO 300 Mitigation of Radiation-Induced Lung Injury
Biomarkers serve a number of purposes during drug development including defining the natural history of injury/disease, serving as a secondary endpoint or trigger for intervention, and/or aiding in the selecti...
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Article
Open AccessLipidomic dysregulation within the lung parenchyma following whole-thorax lung irradiation: Markers of injury, inflammation and fibrosis detected by MALDI-MSI
Radiation-induced lung injury (RILI) is a delayed effect of acute radiation exposure that can limit curative cancer treatment therapies and cause lethality following high-dose whole-thorax lung irradiation (WT...
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Article
Open AccessCol1a1+ perivascular cells in the brain are a source of retinoic acid following stroke
Perivascular stromal cells (PSCs) are a recently identified cell type that comprises a small percentage of the platelet derived growth factor receptor-β+ cells within the CNS perivascular space. PSCs are activ...
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Article
Development and validation of a LC-MS/MS assay for quantitation of plasma citrulline for application to animal models of the acute radiation syndrome across multiple species
The potential risk of a radiological catastrophe highlights the need for identifying and validating potential biomarkers that accurately predict radiation-induced organ damage. A key target organ that is acute...
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Article
Automated Lipid A Structure Assignment from Hierarchical Tandem Mass Spectrometry Data
Infusion-based electrospray ionization (ESI) coupled to multiple-stage tandem mass spectrometry (MS n ) is a standard methodology for investigating lipid A structural diversity (...
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Article
Comprehensive structure characterization of lipid a extracted from Yersinia pestis for determination of its phosphorylation configuration
We report on comprehensive structure characterization of lipid A extracted from Yersinia pestis (Yp) for determination of its phosphorylation configuration that was achieved by combining the methods of molecular ...
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Article
Electron capture in spin-trap capped peptides. An experimental example of ergodic dissociation in peptide cation-radicals
Electron capture dissociation was studied with tetradecapeptides and pentadecapeptides that were capped at N-termini with a 2-(4′-carboxypyrid-2′-yl)-4-carboxamide group (pepy), e. g., pepy-AEQLLQEEQLLQEL-NH2, pe...
