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Article
Open AccessOptimized protocol for whole organ decellularization
The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that ...
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Article
Neuronal apoptosis following human brain injury
Neuronal apoptosis has been investigated in paraffin-embedded brain tissue from 103 individuals who had sustained blunt head injury by use of the in situ nick translation (ISNT) technique. In order to provide ...
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Article
Detection of cell death in human skin wounds of various ages by an in situ end labeling of nuclear DNA fragments
The time-dependent appearance of signs of cell death was investigated in human skin wounds using in situ end labeling of DNA fragments (ISEL). In the dermal layer an average of not more than 0.3 positively st...
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Article
Immunohistochemical analysis of markers for different macrophage phenotypes and their use for a forensic wound age estimation
A total of 117 vital skin wounds (post infliction intervals between a few seconds and 7 months), 20 postmortem wounds and skin specimens with beginning or advanced signs of putrefaction were investigated. Diff...
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Article
Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH)
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial...
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Article
Localization of tenascin in human skin wounds —An immunohistochemical study
A total of 56 surgically treated human skin wounds with a wound age between 8h and 7 months were investigated. Tenascin was visualized by immunohistochemistry and appeared first in the wound area pericellularl...
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Article
Analysis of the immunohistochemical localization of collagen type III and V for the time-estimation of human skin wounds
Collagen type III and V were visualized immunohistochemically in 79 surgically treated human skin wounds with a wound age between 8 h and 2.5 months. Network-like structures positively staining for collagen ty...
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Article
The immunohistochemical localization of alpha1-antichymotrypsin and fibronectin and its meaning for the determination of the vitality of human skin wounds
A total of 39 vital human skin wounds (20 cases with short survival times ranging from a few seconds to approximately 30 min and 19 cases with wound ages between 50 min and 13 days) were investigated. Alpha,-a...
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Article
The time-dependent expression of keratins 5 and 13 during the reepithelialization of human skin wounds
The time-dependent reepithelialization of 55 human surgical skin wounds with a wound age between 8h and more than 2 months was investigated by the immunohistochemical localization of cytokeratins 5 and 13. A c...
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Article
The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds
A total of 77 human skin wounds with a post-infliction interval between 3 h and 7 months were investigated and the proliferation marker antigen Ki 67 was visualized in paraffin sections using a specific monocl...
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Article
Immunohistochemical localization of collagen types I and VI in human skin wounds
A total of 74 human skin wounds were investigated and collagen types I and VI were localized in the wound area by immunohistochemistry. Collagen type I appeared in the form of ramifying string-like structures ...
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Article
Time-dependent appearance of myofibroblasts in granulation tissue of human skin wounds
Human skin wounds (66) inflicted between 20 h and 7 months prior to biopsy were studied. In order to identify the type of cellular differentiation of the fibroblastic cells in the granulation tissue, alpha-smo...
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Article
The time-dependent rearrangement of the epithelial basement membrane in human skin wounds —immunohistochemical localization of Collagen IV and VII
In 62 human skin wounds (surgical wounds, stab wounds and lacerations after surgical treatment) we analyzed the immunohistochemical localization of collagen IV in the epithelial basement membrane. In 27 of the...
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Article
Time-dependent pericellular expression of collagen type IV, laminin, and heparan sulfate proteoglycan in myofibroblasts
Human skin wounds (n = 62) with a wound age between 5 h and 6 weeks were investigated. The appearance of cell-associated pericellular basement membrane components collagen type IV, laminin and heparan sulfate pro...
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Article
Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds
We analyzed the distribution of fibronectin in routinely embedded tissue specimens from 53 skin wounds and 6 postmortem wounds. In postmortem wounds a faint but focal positive staining was exclusively found at...
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Chapter and Conference Paper
Die Knochenheilung — ein immunreaktives Geschehen?
Der Einfluß immunologischer Faktoren auf Vorgänge bei der Knochenheilung ist bislang weitgehend ungeklärt: Einerseits wird Immunreaktionen nach ossären Allo-Transplantaten (Tx) eine Verschlechterung der Tx-Ink...
