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  1. No Access

    Article

    Differential requirements of two insect cell lines for growth in serum-free medium

    The development of a serum-free medium that supports the growth of cells from a Spodoptera frugiperda and a Lymantria dispar cell line is reported. A yeast hydrolysate provided the B-vitamin complex, and a combin...

    J. L. Vaughn, F. Fan in In Vitro Cellular & Developmental Biology - Animal (1997)

  2. No Access

    Article

    Lampyridae (Coleoptera): A plethora of mollicute associations

    Beetles (Coleoptera) harbor many species ofAcholeplasma andSpiroplasma (division Tenericutes, class Mollicutes). Mollicutes were isolated from guts and/or hemocoels of firefly beetles (Lampyridae) from the United...

    K. J. Hackett, R. F. Whitcomb, J. G. Tully, J. E. Lloyd… in Microbial Ecology (1992)

  3. No Access

    Article

    Use of commercial serum replacements for the culture of insect cells

    Two commercially available serum replacements developed for use in the culture of hybridoma and other mammalian cells were tested for their suitability as replacements for fetal bovine serum in insect cell cul...

    J. L. Vaughn, F. Fan in In Vitro Cellular & Developmental Biology (1989)

  4. No Access

    Article

    Effect of aluminum chloride and zinc sulfate onAutographa californica nuclear polyhedrosis virus (ACNPV) replication in cell culture

    When IPL-Sf-21AE III continuous insect cell line was grown and maintained in IPL-41 insect cell culture medium supplemented with 16µM of AlCl3 or 0.24µM of ZnSO4·7H2O, or both metallic salts, and then infected wi...

    S. A. Weiss, G. C. Smith, J. L. Vaughn, E. M. Dougherty, G. J. Tompkins in In Vitro (1982)

  5. Article

    Improved method for the production of insect cell cultures in large volume

    S. A. Weiss, G. C. Smith, S. S. Kalter, J. L. Vaughn in In Vitro (1981)

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  6. No Access

    Article

    Improved method for the production of insect cell cultures in large volume

    An improved semiautomated procedure for growing a cell line from the fall armyworm,Spodoptera frugiperda, under optimal conditions, in a stationary large scale culture, is described in detail. Complete supplement...

    S. A. Weiss, G. C. Smith, S. S. Kalter, J. L. Vaughn in In Vitro (1981)

  7. No Access

    Article

    The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera; noctuidae)

    The history and characteristics of two cell lines developed from primary explants of pupal tissue from the insect,Spodoptera frugiperda (J. E. Smith), are described. One cell line, IPLB-SF-21, was developed with ...

    J. L. Vaughn, R. H. Goodwin, G. J. Tompkins, P. McCawley in In Vitro (1977)

  8. No Access

    Article

    Fatty acid profiles of cells from the insect cell line iprl-21 (Spodoptera frugiperda) and of the tissue culture medium after repeated use

    When IPL-1 medium was used for three serial incubations of cells of the IPRL-21 insect cell line (Spodoptera frugiperda, J. E. Smith) at least 23 fatty acids were identified from the media and/or from the cells. ...

    S. J. Louloudes, J. L. Vaughn, K. A. Dougherty in In Vitro (1973)

  9. No Access

    Chapter and Conference Paper

    Introduction

    Within the last five years the number and variety of insect cell lines has increased rapidly; approximately 20 different cell lines were discussed in the preceeding chapter. As the availability of insect cell ...

    J. L. Vaughn in Arthropod Cell Cultures and Their Application to the Study of Viruses (1971)

  10. No Access

    Chapter and Conference Paper

    The Uptake of Free and Serum-Bound Sterols by Insect Cells in Vitro

    The importance of sterols in insect nutrition has been accepted for some time, and all synthetic diets for insects contain one or more sources of these compounds (Smith, 1966). The sterols are necessary because, ...

    J. L. Vaughn, S. J. Louloudes, K. Dougherty in Arthropod Cell Cultures and Their Applicat… (1971)