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Article
Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus
The operational capacity of a duplex RT-PCR method for simultaneous detection of Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV) has been established by nine European laboratories. A total of 5...
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Article
Determination of the taxonomic position and characterization of yam mosaic virus isolates based on sequence data of the 5′-terminal part of the coat protein cistron
The sequences of the N-terminal part of the coat protein cistron from six isolates of yam mosaic virus (YMV-TOG, YMV-COT, YMV-12, YMV-CAR, YMV-BU1 and YMV-BU2) were determined. The analysis of the deduced amin...
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Article
Molecular evidence that the whitefly-transmitted sweetpotato mild mottle virus belongs to a distinct genus of thePotyviridae
Complementary DNA representing 2 108 nucleotides at the 3′ end of the genomic RNA of the whitefly-transmitted sweetpotato mild mottle virus (SPMMV) was cloned after PCR. Sequence analysis revealed an open read...
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Article
The complete nucleotide sequences of the coat protein cistron and the 3′ non-coding region of a newly-identified potyvirus infecting sweetpotato, as compared to those of sweetpotato feathery mottle virus
Complementary DNA representing 728 nucleotides of the 3′ end of the genomic RNA of sweetpotato virus G (SPV-G), a newly-identified potyvirus infecting sweetpotato, was cloned and sequenced. This sequence was c...
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Article
Use of VIRAZOLE® to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw.
Odontoglossum Ringspot Virus has been eradicated from Cymbidium Sw. through chemotherapy based on incorporation of ribavirin (VIRAZOLE®) into the in vitro culture medium of protocorms. Applications of the virusta...