23 Result(s)
within
J. Hescheler
Cell Biology
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Article
Open AccessCav2.3 R-type calcium channels: from its discovery to pathogenic de novo CACNA1E variants: a historical perspective
So-called pharmacoresistant (R-type) voltage-gated Ca2+ channels are structurally only partially characterized. Most of them are encoded by the CACNA1E gene and are expressed as different Cav2.3 splice variants (...
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Article
Open AccessNeuronal developmental gene and miRNA signatures induced by histone deacetylase inhibitors in human embryonic stem cells
Human embryonic stem cells (hESCs) may be applied to develop human-relevant sensitive in vitro test systems for monitoring developmental toxicants. The aim of this study was to identify potential developmental to...
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Article
Open AccessFam40b is required for lineage commitment of murine embryonic stem cells
FAM40B (STRIP2) is a member of the striatin-interacting phosphatase and kinase (STRIPAK) complex that is involved in the regulation of various processes such as cell proliferation and differentiation. Its role...
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Article
Engineered embryonic stem cells for regnerative nedicine
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Article
The SRC family kinase LYN redirects B cell receptor signaling in human SLP65-deficient B cell lymphoma cells
SLP65 represents a critical component in (pre-) B cell receptor signal transduction but is compromised in a subset of pre-B cell-derived acute lymphoblastic leukemia. Based on these findings, we investigated (...
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Article
Outwards currents in embryonic stem cell-derived cardiomyocytes
The aim of the present study was to investigate the expression and functional role of outwards currents during the early stages of cardiomyogenesis. The predominant repolarizing current in early-stage, embryon...
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Article
Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture
Three-dimensional cell aggregates (embryoid bodies, EBs) containing clusters of spontaneously beating cardiomyocytes were derived from permanent mouse embryonic stem (ES) cells. Extracellular recordings of th...
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Chapter and Conference Paper
Embryonic Stem Cell Derived Cardiogenesis and Myogenesis
Permanent cell lines of embryonic stem (ES) cells established from totipotent cells of the mouse embryo provide one of the most important cellular systems for developmental biologists. ES cells offer a suitabl...
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Article
Abstract of the 68th Meeting (Spring Meeting) 6–9 March 1990, Heidelberg
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Article
Calcium currents of neuroblastoma × glioma hybrid cells after cultivation with dibutyryl cyclic AMP and nickel
The long-term modulation of calcium (Ca2+) currents (I Ca) was studied in 108CC15 neuroblastoma × glioma hybrid (N×G) cells grown under various culture conditions. The following results were obtained:
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Article
Regular and chaotic behaviour of cardiac cells stimulated at frequencies between 2 and 20 Hz
We measured the non-linear dynamics of cardiac action potentials by varying the stimulation frequency from 2 to 20 Hz and obtained the following results: (i) When the fast Na+ current initiated the action potenti...
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Chapter and Conference Paper
Involvement of Pertussis Toxin-Sensitive G-Proteins in the Modulation of Voltage-Dependent Ca2+ Channels by Extracellular Signals
Heterotrimeric guanine nucleotide-binding proteins (G-proteins; see Chap. 9, this Vol.) are located at the inner face of the plasma membrane. They act as transducer molecules by coupling transmembranous recept...
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Article
A possible physiological role of the Ca-dependent protease calpain and its inhibitor calpastatin on the Ca current in guinea pig myocytes
The decrease (‘run-down’) of the L-type Ca2+-current during long-lasting recordings with the whole-cell patch-clamp technique was examined in guinea pig ventricular myocytes. We have tested whether proteolysis is...
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Article
Effects of a protein phosphatase inhibitor, okadaic acid, on membrane currents of isolated guinea-pig cardiac myocytes
The effects of a protein phosphatase inhibitor, okadaic acid (OA), were studied on membrane currents of isolated myocytes from guinea-pig cardiac ventricle. The whole-cell Ca2+ current (I Ca) was recorded as peak...
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Article
“Run-down” of the Ca current during long whole-cell recordings in guinea pig heart cells: role of phosphorylation and intracellular calcium
We examined by a statistical approach the decrease of the Ca current (“run-down”) during long-lasting recordings with the whole-cell patch-clamp technique in guinea pig ventricular myocytes. The results are as...
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Article
On the mechanism of histamine induced enhancement of the cardiac Ca2+ current
In guinea pig ventricular myocytes, the effect of histamine on the slow Ca2+ current (ICa) was studied and the following results were obtained: (1) Superfusion of cells with histamine resulted in a dose-dependent...
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Article
Changes of membrane currents in cardiac cells induced by long whole-cell recordings and tolbutamide
Single isolated myocytes were obtained from the ventricles of adult guinea pig hearts. The whole-cell recording configuration of the patch-clamp technique was used to measure membrane currents. A decrease (run...
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Article
The protein-specific phosphatase 1 antagonizes the β-adrenergic increase of the cardiac Ca current
In isolated ventricular cells from the adult guinea pig heart the slow Ca current was recorded during β-adrenergic stimulation and during cell dialysis with a protein-specific phosphatase-1 (PPase-1).
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Article
Modulation of Ca current during the phosphorylation cycle in the guinea pig heart
The calcium current (I Ca) in the heart is increased by phosphorylation of a protein which is part of, or close to, the Ca channel. The phosphorylation is catalysed by cAMP-dependent protein kinase (cAMP-PK). The...
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Article
On the mechanism of muscarinic inhibition of the cardiac Ca current
The mechanism of muscarinic inhibition of the Ca-current (I Ca) was studied in ventricular myocytes of guinea pig hearts and the following results were obtained. 1. Acetylcholine (ACh) in concentrations up to 10−...
