![Loading...](https://link.springer.com/static/c4a417b97a76cc2980e3c25e2271af3129e08bbe/images/pdf-preview/spacer.gif)
-
Article
Polarity of actin at the leading edge of cultured cells
VERTEBRATE non-muscle cells are known to contain considerable amounts of actin and myosin1–3, but the mechanisms underlying their motility have yet to be elucidated. Various theories have been proposed1,4–7 to ex...
-
Book
-
Chapter
Tumorigenicity, Actin Cables and Gene Expression in Mouse CLID × CHO Cell Hybrids
There is pressing need to search for assays of transformation that could be used to assess malignancy or tumorigenicity in cultured cells. A variety of reports have appeared correlating abnormal properties of ...
-
Chapter
Microinjection of Somatic Cells with Micropipettes and PEG-Erythrocyte Ghost Mediated Microinjection
The development of techniques to introduce macromolecules into living somatic cells such as the direct microinjection with micropipettes (1, 2), the red cell mediated microinjection (3–7) and the liposome medi...
-
Chapter
Aspects of Cell Architecture and Locomotion
Three morphologically and biochemically distinct filament types may be recognised in the cytoplasm of eukaryotic cells: actin filaments (or microfilaments), microtubules and 10nm filaments (Fig. 1) Although th...
-
Book
-
Chapter
Expression of Cellular Protein in Normal and Transformed Human Cultured Cells
Malignant transformation of cultured cells is often characterized by changes in growth properties as well as of cell morphology (1–3). These alterations reflect changes in gene expression that develop through ...
-
Article
Changes in the levels of human tropomyosins IEF 52,55, and 56 do not correlate with the loss of actin cables observed in SV 40 transformed MRC-5 fibroblasts
A mouse monoclonal antibody (mAb 1D122G9) raised against human tropomyosin IEF 52 (HeLa protein catalogue number, Mr=35 kd) has been characterized both in terms of specificity and patterns of immunofluorescenc...
-
Chapter
Protein-Electroblotting and Microsequencing in Establishing Integrated Human Protein Databases
Proteins, which are characteristic for a specific state of differentiation, the transformed phenotype or pathological conditions of human cells and tissues were identified by computer analyzed two-dimensional ...
-
Article
TNF-α induces dyscohesion of epithelial cells. Association with disassembly of actin filaments
TNF-α induced, in a time and dose-dependent fashion, cell-cell dissociation (dyscohesion) of endometrial epithelial cells. Within the time frame that dyscohesion was induced, TNF-α, in a dose-dependent fashion...
-
Article
From genomics to proteomics
Proteomics is a modern, rapidly develo** branch of biology aimed at the integral study of genome expression as the diversity of proteins. Proteomics is based on a variety of methods allowing one to isolate, ...