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From genomics to proteomics

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Abstract

Proteomics is a modern, rapidly develo** branch of biology aimed at the integral study of genome expression as the diversity of proteins. Proteomics is based on a variety of methods allowing one to isolate, detect, identify, and to analyze quantitatively thousands of proteins comprising the cellular proteome. Up to date, high-resolution two-dimensional electrophoresis is the key proteomic technology; it allows one to analyze simultaneously the protein composition of cells and tissues and to reveal quantitative and qualitative changes in the synthesis of proteins modulating multiple cell functions. The present status of proteomic technologies, i.e., of 2D electrophoresis, with accompanying methods of identification of proteins at the maps obtained, as well as a brief description of organization of human mouse 2D database suggested for global analysis of genome expression in normal and pathological conditions (http:/biobase.dk/cgi-bin/celis) are discussed.

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Authors and Affiliations

  1. Institute of Medical Biochemistry and Danish Human Genome Center, University of Orhus, Orhus, Denmark

    P. S. Gromov & J. E. Celis

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  1. P. S. Gromov
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Gromov, P.S., Celis, J.E. From genomics to proteomics. Mol Biol 34, 508–520 (2000). https://doi.org/10.1007/BF02759560

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