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  1. No Access

    Article

    Isolation and Characterization of a Serine Protease, Ba III-4, from Peruvian Bothrops atrox venom

    A serine protease from Bothrops atrox (Peruvian specimen’s venom) was isolated in two chromatographic steps in LC molecular exclusion and reverse phase-HPLC. This protein was denominated B...

    L. A. Ponce-Soto, V. L. Bonfim, J. C. Novello, R. Navarro Oviedo… in The Protein Journal (2007)

  2. No Access

    Article

    Structural and Functional Properties of Cr 5, a New Lys49 Phospholipase A2 Homologue Isolated from the Venom of the Snake Calloselasma rhodostoma

    Cr 5 PLA2 homologous (K49) was isolated from Calloselasma rhodostoma venom in only one chromatographic step in reverse phase HPLC (RP-HPLC) (on μ-Bondapack C-18). A molecular mass of 13.965 Da was determined by M...

    V. L. Bonfim, L. A. Ponce-Soto, J. C. Novello, S. Marangoni in The Protein Journal (2006)

  3. No Access

    Article

    Determination of Primary Structure of Two Isoforms 6-1 and 6-2 PLA2 D49 from Bothrops jararacussu Snake Venom and Neurotoxic Characterization Using in vitro Neuromuscular Preparation

    In this paper we reported the purification, the biological characterization and the amino acid sequence of two new isoforms basic 6-1 (Bj-IV) and 6-2 (Bj-V) PLA2 D49 purified from the Bothrops jararacussu venom

    L. A. Ponce-Soto, V. L. Bonfim, L. Rodrigues-Simioni, J. C. Novello… in The Protein Journal (2006)

  4. No Access

    Article

    Isolation and Characterization of a Lectin from Annona muricata Seeds

    A lectin with a high affinity for glucose/mannose was isolated from Annona muricata seeds (Annonaceae) by gel filtration chromatography on Sephacryl S-200, ion exchange chromatography on a DEAE SP-5 PW column, an...

    D. C. S. Damico, M. G. M. Freire, V. M. Gomes, M. H. Toyama… in Journal of Protein Chemistry (2003)

  5. No Access

    Article

    Biochemical Characterization of a Lectin from Delonix regia Seeds

    A lectin from Delonix regia (DRL) seeds was purified by gel filtration on Sephadex G-100 followed by ion-exchange chromatography on diethylaminoethyl-Sepharose and reverse-phase high-performance liquid chromatogr...

    S. C. Pando, M. L. R. Macedo, M. G. M. Freire, M. H. Toyama… in Journal of Protein Chemistry (2002)

  6. No Access

    Article

    Isolation and Preliminary Enzymatic Characterization of a Novel PLA2 from Crotalus durissus collilineatus Venom

    A crotoxin homolog was purified from the Crotalus durissus collilineatus venom using molecular exclusion and reverse-phase HPLC. This crotoxin contained one PLA2 (Cdcolli III F6) and four crotapotin isoforms, whe...

    L. A. Ponce-Soto, M. H. Toyama, S. Hyslop, J. C. Novello… in Journal of Protein Chemistry (2002)

  7. No Access

    Article

    Structural and Functional Characterization of Basic PLA2 Isolated from Crotalus durissus terrificus Venom

    The venom of Crotalus durissus terrificus was fractionated by reverse-phase HPLC to obtain crotapotins (F5 and F7) and PLA2 (F15, F16, and F17) of high purity. The phospholipases A2 (PLA2s) and crotapotins showed...

    D. G. Oliveira, M. H. Toyama, J. C. Novello… in Journal of Protein Chemistry (2002)

  8. No Access

    Article

    Isolation and Enzymatic Characterization of a Basic Phospholipase A2 from Bothrops jararacussu Snake Venom

    A novel basic phospholipase A2 (PLA2) isoform was isolated from Bothrops jararacussu snake venom and partially characterized. The venom was fractionated by HPLC ion-exchange chromatography in ammonium bicarbonate...

    V. L. Bonfim, M. H. Toyama, J. C. Novello, S. Hyslop… in Journal of Protein Chemistry (2001)

  9. No Access

    Article

    Biochemical Characterization and N-Terminal Sequences of Two New Trypsin Inhibitors from Copaifera langsdorffii Seeds

    Two new trypsin inhibitors, TDI-I and TDI-II, were purified from the seeds of the native Brazilian tree Copaifera langsdorffii (Caesalpinoideae, Leguminosae). The purification procedure involved ammonium sulfa...

    J. A. Silva, M. L. R. Macedo, J. C. Novello, S. Marangoni in Journal of Protein Chemistry (2001)

  10. No Access

    Article

    Enzymatic Characterization of a Novel Phospholipase A2 from Crotalus durissus cascavella Rattlesnake (Maracambóia) Venom

    The PLA2 and crotapotin subunits of crotoxin from Crotalus durissus cascavella venom were purified by a combination of HPLC molecular exclusion (Protein Pack 300SW column) and reverse-phase HPLC (RP-HPLC). Tricin...

    D. G. Beghini, M. H. Toyama, S. Hyslop, L. Sodek… in Journal of Protein Chemistry (2000)

  11. No Access

    Article

    The Amino Acid Sequence of Bothropstoxin-II, an Asp-49 Myotoxin from Bothrops jararacussu (Jararacucu) Venom with Low Phospholipase A2 Activity

    The complete amino acid sequence of bothropstoxin-II (BthTX-II), a myotoxin isolated from Bothrops jararacussu snake venom, is reported. The results show that BthTX-II is an Asp-49 phospholipase A2 (PLA2)-like pr...

    M. F. Pereira, J. C. Novello, A. C. O. Cintra, J. R. Giglio… in Journal of Protein Chemistry (1998)