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Article
Biochemical and physiological changes produced by Azotobacter chroococcum (INIFAT5 strain) on pineapple in vitro-plantlets during acclimatization
This study highlights some of the effects of the application of Azotobacter chroococcum (INIFAT5 strain) on in vitro-pineapple plantlets during acclimatization. The bacteria were sprayed immediately after transp...
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Article
Integrated criteria to identify the best treatment in plant biotechnology experiments
Plant scientists usually record many indicators in their experiments. The common statistical management involves univariate analyses. Such analyses generally create a split picture of the effects of experiment...
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Article
Micropropagation of Hohenbergia penduliflora (A. Rich.) Mez. for sustainable production of plant proteases
Hohenbergia penduliflora (A. Rich.) Mez. inhabits the protected ecological area of Cunagua Highland, Ciego de Ávila, Cuba. The availability of this plant for experimental purposes is exce...
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Article
Penduliflorain I: A Cysteine Protease Isolated from Hohenbergia penduliflora (A.Rich.) Mez (Bromeliaceae)
Penduliflorain I, a new plant endopeptidase, was isolated and characterized from Hohenbergia penduliflora. Crude extract was obtained from stems. A partially purified enzyme preparation was obtained by ethanol pr...
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Article
Reply to RE: Use of regression analysis in plant cell, tissue and organ culture experiments (44(3): 229–232)
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Article
Open AccessEstimated economic benefit of double-haploid technique for peruvian barley growers and breeders
Although barley is extensively grown in the Peruvian highlands as a food crop, agronomic and quality performance are poor. In addition, there are few reports of the successful application of modern technologie...
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Article
Use of regression analysis in plant cell, tissue, and organ culture experiments
Several authors have suggested that plant biotechnologists perform regression or trend analysis to compare means of related quantitative treatments (e.g., doses of inositol). The present paper compares two sta...
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Article
Effect of sucrose, inorganic salts, inositol, and thiamine on protease excretion during pineapple culture in temporary immersion bioreactors
Although pineapple plants have been found to produce proteases ex vitro, most of the biotechnological investigations of this crop have been focused on propagation. The procedure involving the use of temporary imm...
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Article
Improved Technique for Rapid and Non-Destructive In Vitro Differentiation between Resistant and Susceptible Banana Clones of Fusarium Oxysporum f. sp. Cubense
Culture filtrates of Fusarium oxysporum f. sp. cubense were applied to field-grown banana leaves of susceptible and resistant clones. The difference in leaf lesions, measured after 48 h, varied from 13 to 51 mm2 ...
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Article
Protease excretion during pineapple micropropagation in temporary immersion bioreactors
Biotechnology has become an important tool to produce plant secondary metabolites and proteases are among them. Although pineapple plants have been found to produce proteases, most of the biotechnological inve...
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Article
Bioassay for in vitro differentiation of pineapple cultivar resistance levels to heart rot disease
An in vitro bioassay to differentiate pineapple plant resistance levels to Phytophthora nicotianae var. parasitica (heart rot disease) is deseribed here. Conditions to cause death of in vitro-cultured plants were...
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Article
Field performance of temporary immersion bioreactor-derived sugarcane plants
The temporary immersion bioreactor has been found to be an important tool for sugarcane micropropagation, allowing higher shoot formation rates and cost reduction. This research was conducted to demonstrate th...
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Article
Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems
A procedure for the mass propagation of pineapple plants (Ananas comosus L. Merr) using a temporary immersion technique is described. This procedure involved three distinct phases in the automated temporary imme...
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Chapter
Temporary Immersion for Plant Tissue Culture
Mass propagation of plants by tissue culture is labour-intensive and costly. Gelling agents have many drawbacks: they are not an inert medium component and do not enable easy automation. So liquid culture syst...
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Chapter and Conference Paper
Experimental Nerve Anastomoses with Fibrin Adhesive in a Rabbit Model
The aim of this study was to determine if the interposition of a 4 mm block of fibrin sealant in the nervus tibialis (internal popliteal nerve) of the rabbit would form a barrier which could stop or interfere ...
