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Article
Open AccessRapid Assessment of Bio-distribution and Antitumor Activity of the Photosensitizer Bremachlorin in a Murine PDAC Model: Detection of PDT-induced Tumor Necrosis by IRDye® 800CW Carboxylate, Using Whole-Body Fluorescent Imaging
Photodynamic therapy (PDT) is a light-based anticancer therapy that can induce tumor necrosis and/or apoptosis. Two important factors contributing to the efficacy of PDT are the concentration of the photosensi...
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Article
Open AccessUpconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking
Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluore...
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Article
Open AccessMultimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury
Traumatic brain injury (TBI) is a devastating event for which current therapies are limited. Stem cell transplantation may lead to recovery of function via different mechanisms, such as cell replacement throug...
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Article
Open AccessClick beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging
The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high. This ...
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Article
Open AccessDeficiency of TLR4 homologue RP105 aggravates outward remodeling in a murine model of arteriovenous fistula failure
Arteriovenous access dysfunction is a major cause of morbidity for hemodialysis patients. The pathophysiology of arteriovenous fistula (AVF) maturation failure is associated with inflammation, impaired outward...
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Article
Targeted nanoparticles for the non-invasive detection of traumatic brain injury by optical imaging and fluorine magnetic resonance imaging
Necrosis is a form of cell death that occurs only under pathological conditions such as ischemic diseases and traumatic brain injury (TBI). Non-invasive imaging of the affected tissue is a key component of nov...
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Article
Open AccessIdentification of receptor-type protein tyrosine phosphatase μ as a new marker for osteocytes
Osteocytes are the predominant cells in bone, where they form a cellular network and display important functions in bone homeostasis, phosphate metabolism and mechanical transduction. Several proteins strongly...
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Article
An orthotopic mouse model for chondrosarcoma of bone provides an in vivo tool for drug testing
Chondrosarcoma is a malignant cartilaginous tumor of the bone. Recently, mutations in isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2) were identified in central chondrosarcomas. As chondro...
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Article
In Vivo Fluorescence Imaging of IgG1 Aggregates After Subcutaneous and Intravenous Injection in Mice
To monitor the biodistribution of IgG1 aggregates upon subcutaneous (SC) and intravenous (IV) administration in mice and measure their propensity to stimulate an early immune response.
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Article
Open AccessAutomated Bone Volume and Thickness Measurements in Small Animal Whole-Body MicroCT Data
Quantification of osteolysis is crucial for monitoring treatment effects in preclinical research and should be based on MicroCT data rather than conventional 2D radiographs to obtain optimal accuracy. However,...
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Protocol
Bioluminescence Imaging of Bone Metastasis in Rodents
Optical imaging is a valuable technique for visualizing and quantifying biological processes in living organisms. Optical imaging can be divided into two main imaging modalities: bioluminescence imaging and f...
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Article
Image-guided tumor resection using real-time near-infrared fluorescence in a syngeneic rat model of primary breast cancer
Tumor involvement of resection margins is found in a large proportion of patients who undergo breast-conserving surgery. Near-infrared (NIR) fluorescence imaging is an experimental technique to visualize cance...
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Article
Open AccessOptical Image-guided Surgery—Where Do We Stand?
In cancer surgery, intra-operative assessment of the tumor-free margin, which is critical for the prognosis of the patient, relies on the visual appearance and palpation of the tumor. Optical imaging technique...
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Article
Open AccessMolecular imaging of tumor angiogenesis using αvβ3-integrin targeted multimodal quantum dots
Molecular imaging of angiogenesis is urgently needed for diagnostic purposes such as early detection, monitoring of (angiostatic) therapy and individualized therapy. Multimodality molecular imaging is a promis...
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Article
Advances in optical imaging and novel model systems for cancer metastasis research
Research into the genetic and physiological interactions of tumours with their host environment requires in vivo assays to address molecular expression patterns and function. In recent years much of this work ...
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Article
Open AccessFunctional imaging of multidrug resistance in an orthotopic model of osteosarcoma using 99mTc-sestamibi
The purpose of this work was the development of an orthotopic model of osteosarcoma based on luciferase-expressing tumour cells for the in vivo imaging of multidrug resistance (MDR) with 99mTc-sestamibi.
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Article
Open AccessEfficient in vivoknock-down of estrogen receptor alpha: application of recombinant adenovirus vectors for delivery of short hairpin RNA
Adenovirus (Ad) mediated gene transfer is a well-established tool to transiently express constructs in livers of mice in vivo. In the present study, we determined the specificity and efficiency of Ad vectors expr...
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Article
In vivo imaging of transcriptionally active estrogen receptors
Through intracellular receptors, estrogens control growth, differentiation and function of not only reproductive tissues, but also other systems1,2,3. Estrogen receptors are ligand-dependent transcription factors...
