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Chapter
Electrochemical Preparation of Enzyme-Collagen Membrane and Its Application
The electrochemical preparation of membrane from fibrous protein such as collagen has been developed in our laboratory since 1966 (1,2,3).
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Article
Photocontrol of lactate dehydrogenase-spiropyran collagen membrane
Collagen fibrils were modified with β-l-[3,3-dimethyl-6′-nitrospiro-(indoline-2,2′-2H-benzopyran)] propionic anhydride. The spiropyran collagen membrane showed reverse photochromism. Lactate dehydrogenase (LDH...
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Article
Lysis of bacteria in aerosol by immobilized enzymes
Bacteriolytic enzymes (protease and β-1,3-glucanase) produced byAchromobacter lunatus were immobilized in collagen membrane. Approximately 20% of intactBacillus subtilis in aerosol was lyzed by the bacteriolytic ...
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Article
Continuous neutral lipid determination with lipase-collagen membrane reactor
Lipase (Pseudomonas sp.) was immobilized in collagen membrane and used for the measurement of neutral lipids. The determination system for lipids consisted of a lipase-collagen membrane reactor and glass elect...
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Article
Immobilization of AMP-deaminase in acrylamide-glycidylmethacrylate copolymer
The enzyme AMP-deaminase was entrapped in an acrylamide-glycidylmethacrylate copolymer. No leakage of AMP-deaminase was observed from the copolymer. The optimum pH of native AMP-deaminase was 5.6. The pH was d...
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Article
Photocontrolled binding of cytochrome C to immobilized spiropyran
Agarose gel was modified with polyethyleneimine and β-l-[3,3-dimethyl-6’-nitrospiro-(indoline-2,2’-2H-benzopyran)] propionic anhydride and the binding of cytochrome c was investigated. Cytochrome c was not ret...
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Article
A new microbial electrode for BOD estimation
A new microbial electrode using immobilizedClostridium butyricum was prepared for biochemical oxygen demand (BOD) estimation of wastewaters. The current of the electrode was decreased with time until a steady sta...
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Article
Preparation, properties, andin vitro thrombogenic characterization of urokinase-collagen membrane
Urokinase (EC 3.4.4.a) was immobilized on collagen membrane. The urokinase-collagen membrane gave a flat pH profile from 7.5 to 9.5. It was more stable against heat than native urokinase. Furthermore, the stab...
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Article
α-Amylase production by immobilized whole cells of Bacillus subtilis
Whole cells of Bacillus subtilis were immobilized in polyacrylamide gel prepared from 5% total acrylamide (85% acrylamide and 15% N,N′-methylenebisacrylamide). Production of α-amylase by the immobilized whole cel...
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Article
Amperometric estimation of BOD by using living immobilized yeasts
A microbial electrode consisting of immobilized living whole cells of yeasts, porous membrane and an oxygen electrode was prepared for continuous estimation of biochemical oxygen demand (BOD). Immobilized Trichos...
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Article
Glucose sensor using immobilized whole cells of Pseudomonas fluorescens
Whole cells of Pseudomonas fluorescens which utilized mainly glucose were immobilized in collagen membrane. The microbial electrode consisted of a bacteria-collagen membrane and an oxygen electrode was developed ...
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Chapter
Application of Photosensitive Immobilized α-Chymotrypsin to Synthetic Reaction
Hydrolytic enzymes can be used to catalyze the synthesis of the compounds that they normally hydrolyze. Substances which undergo reversible color formation under light irradiation are called photochromic compo...
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Chapter
Microbial Sensors for Ethyl Alcohol, Acetic Acid and Ammonia
Microbial sensors consisting of immobilized whole cells and electrochemical devices have been developed for the determination of raw materials, cell population and products in microbial media (1,2,3). Microbia...
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Chapter
Electrochemical Determination of Lipids in Serum
The determination of lipids in serum is very important in clinical analysis. Quantitative determination of lipids can be carried out colorimetrically; however, pretreatment such as lipid extraction and separat...
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Chapter
Biochemical Energy Conversion System
Considerable world-wide interest has arisen in development of new energy conversion systems. Utilization of biomass and waste water is attractive for energy production. The authors succeeded in stabilization o...
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Article
Enhancement of penicillin acylase activity by cultivating immobilized Kluyvera citrophila
Whole cells of Kluyvera citrophila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%–70% of native cells. When the immobilized cells were continuously cult...
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Article
Electrochemical determination of cell populations
Determination of cell populations was carried out using the potentiostatic systems. The system was constructed from two platinum electrodes and a saturated calomel electrode. The anode of a reference system wa...
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Article
A specific microbial sensor for formic acid
A microbial sensor consisting of immobilized Clostridium butyricum, two gas permeable Teflon membranes and fuel cell type electrode was suitable for the determination of formic acid. When the sensor was inserted ...
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Article
A rapid electrochemical method for assimilation test of microorganisms
A microbial electrode consisting of the immobilized microorganisms to be tested and an oxygen electrode was used to study the assimilation characteristics of microorganisms. When a sample solution containing a...
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Article
Production of 1,2-epoxyalkanes from 1-alkenes by Nocardia corallina B-276
A gaseous hydrocarbon-assimilating microorganism, Nocardia corallina, grew on 1-alkenes (C3, C4 and C13 to C18) and produced corresponding 1,2-epoxyalkanes. One of the products, 1,2-epoxytetradecane, had a positi...
