24 Result(s)
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Article
α-Amylase production by immobilized whole cells of Bacillus subtilis
Whole cells of Bacillus subtilis were immobilized in polyacrylamide gel prepared from 5% total acrylamide (85% acrylamide and 15% N,N′-methylenebisacrylamide). Production of α-amylase by the immobilized whole cel...
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Article
Amperometric estimation of BOD by using living immobilized yeasts
A microbial electrode consisting of immobilized living whole cells of yeasts, porous membrane and an oxygen electrode was prepared for continuous estimation of biochemical oxygen demand (BOD). Immobilized Trichos...
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Article
Glucose sensor using immobilized whole cells of Pseudomonas fluorescens
Whole cells of Pseudomonas fluorescens which utilized mainly glucose were immobilized in collagen membrane. The microbial electrode consisted of a bacteria-collagen membrane and an oxygen electrode was developed ...
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Article
Enhancement of penicillin acylase activity by cultivating immobilized Kluyvera citrophila
Whole cells of Kluyvera citrophila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%–70% of native cells. When the immobilized cells were continuously cult...
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Article
Electrochemical determination of cell populations
Determination of cell populations was carried out using the potentiostatic systems. The system was constructed from two platinum electrodes and a saturated calomel electrode. The anode of a reference system wa...
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Article
A specific microbial sensor for formic acid
A microbial sensor consisting of immobilized Clostridium butyricum, two gas permeable Teflon membranes and fuel cell type electrode was suitable for the determination of formic acid. When the sensor was inserted ...
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Article
A rapid electrochemical method for assimilation test of microorganisms
A microbial electrode consisting of the immobilized microorganisms to be tested and an oxygen electrode was used to study the assimilation characteristics of microorganisms. When a sample solution containing a...
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Article
Production of 1,2-epoxyalkanes from 1-alkenes by Nocardia corallina B-276
A gaseous hydrocarbon-assimilating microorganism, Nocardia corallina, grew on 1-alkenes (C3, C4 and C13 to C18) and produced corresponding 1,2-epoxyalkanes. One of the products, 1,2-epoxytetradecane, had a positi...
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Article
A photochemical fuel cell system using Anabaena N-7363
A blue-green algae, Anabaena N-7363, was immobilized in 2% agar gel. The hydrogen productivity of the immobilized algae was three times higher than that of free algae. The maximum hydrogen production rate by the ...
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Article
Microbial sensor system which usesMethylomonas sp. for the determination of methane
A microbial sensor system was prepared for the detemination of methane. This microbial sensor consisted of immobilizedMethylomonas sp. and an oxygen electrode. When sample gas containing methane was applied to th...
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Article
Amperometric determination of viable cell numbers based on sensing microbial respiration
Viable cell numbers were determined with an electrode system composed of a membrane filter for retaining microorganisms and an oxygen electrode. The principle of the cell number determination is based on sensi...
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Article
Stress sensitive glucose oxidase-nylon membrane
Glucose oxidase (GOD) was immobilized on a nylon membrane. No activity of the GOD-nylon membrane was observed under normal conditions but it appeared when the membrane was mechanically stretched. A linear rela...
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Article
Ultrasound sensitive enzyme-membrane
Glucose oxidase (GOD) was immobilized on an acetylcellulose membrane filter (pore size: 0.45 μm) with dipalmitoyl phosphatidyl-choline (DPPC). The activity of the GOD-DPPC membrane appeared during ultrasonic i...
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Article
Hydrogen production from glucose by immobilized growing cells ofClostridium butyricum
Clostridium butyricum was immobilized in a porous carrier (acetylcellulose filter) with agar. Addition of peptone to the reaction mixture increased the hydrogen productivity from glucose. The number of cells in ...
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Article
Amperometric determination of sodium nitrite by a microbial sensor
A microbial sensor was prepared to determine sodium nitrite. This microbial sensor consisted of immobilized Nitrobacter sp. and an oxygen electrode. When a sample solution containing sodium nitrite was tested, ni...
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Determination of cell numbers of lactic acid producing bacteria by lactate sensor
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Article
Hybrid urea sensor using nitrifying bacteria
A new urea sensor was developed using immobilized nitrifying bacteria isolated from activated sludges and urease. The sensor was composed of a urease-membrane, a cation exchange membrane, and an alkaline bed (...
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Article
Sulfite ion sensor with use of immobilized organelle
The sulfite ion sensor consisted of immobilized microsomes and an oxygen electrode. When the sensor was inserted into the sample solution, a steady-state current was obtained within 10 min. A linear relationsh...
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Article
Isolation and partial characterization of large plasmids in hydrogen-evolving bacterium Clostridium butyricum
The hydrogen-evolving bacterium Clostridium butyricum IFO 3847 was found to carry three plasmids: pSSK1, pSSK2, and pSSK3. The molecular weights of pSSK1, 2 and 3, determined by agarose gel electrophoresis and el...
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Article
Enzyme sensor for hypoxanthine and inosine determination in edible fish
An enzyme sensor for hypoxanthine (Hx) and inosine (HxR), consisting of an enzyme membrane and an oxygen electrode, was constructed, Xanthine oxidase (XO) and nucleoside phosphorylase (NP) were both immobilize...
