31 Result(s)
within
Biochemistry, general
Isao Karube
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Article
Photocontrol of lactate dehydrogenase-spiropyran collagen membrane
Collagen fibrils were modified with β-l-[3,3-dimethyl-6′-nitrospiro-(indoline-2,2′-2H-benzopyran)] propionic anhydride. The spiropyran collagen membrane showed reverse photochromism. Lactate dehydrogenase (LDH...
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Article
Lysis of bacteria in aerosol by immobilized enzymes
Bacteriolytic enzymes (protease and β-1,3-glucanase) produced byAchromobacter lunatus were immobilized in collagen membrane. Approximately 20% of intactBacillus subtilis in aerosol was lyzed by the bacteriolytic ...
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Article
Continuous neutral lipid determination with lipase-collagen membrane reactor
Lipase (Pseudomonas sp.) was immobilized in collagen membrane and used for the measurement of neutral lipids. The determination system for lipids consisted of a lipase-collagen membrane reactor and glass elect...
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Article
Immobilization of AMP-deaminase in acrylamide-glycidylmethacrylate copolymer
The enzyme AMP-deaminase was entrapped in an acrylamide-glycidylmethacrylate copolymer. No leakage of AMP-deaminase was observed from the copolymer. The optimum pH of native AMP-deaminase was 5.6. The pH was d...
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Article
Photocontrolled binding of cytochrome C to immobilized spiropyran
Agarose gel was modified with polyethyleneimine and β-l-[3,3-dimethyl-6’-nitrospiro-(indoline-2,2’-2H-benzopyran)] propionic anhydride and the binding of cytochrome c was investigated. Cytochrome c was not ret...
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Article
A new microbial electrode for BOD estimation
A new microbial electrode using immobilizedClostridium butyricum was prepared for biochemical oxygen demand (BOD) estimation of wastewaters. The current of the electrode was decreased with time until a steady sta...
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Article
Preparation, properties, andin vitro thrombogenic characterization of urokinase-collagen membrane
Urokinase (EC 3.4.4.a) was immobilized on collagen membrane. The urokinase-collagen membrane gave a flat pH profile from 7.5 to 9.5. It was more stable against heat than native urokinase. Furthermore, the stab...
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Article
Recent trends of biosensors in Japan
Remarkable progress has been made in the development of bioelectrochemical sensors, and various kinds of biosensors have been developed and applied in analytical chemistry. Many of these enzyme, microbial, and...
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Chapter
Electric Pulse Accelerated Immunoassay
Variqus antigens including proteins, peptides, drugs and microorganisms are determined by immunoassay. The immunoassay is based on the specific binding reaction of an antigen to its antibody. Immunoreaction re...
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Article
Basic studies of hydrogen evolution by escherichia coli containing a cloned citrobacter freundii hydrogenase gene
Citrobacter freundii genes that complementedEscherichia coli hyd(hydrogenase activity) mutation were cloned in plasmids pCBH4 (6.2 kb) and pCBH6(5.7 kb) (1,2). Hydrogen evolution by the transform...
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Article
Tumor cell detection method using complement-mediated cytolytic reaction and imaging sensor system
A novel tumor-detection system consisting of complementmediated cytolytic reaction and an image processing system was developed for the simple and rapid determination of tumor cells.
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Article
Facile isolation ofEndo-pectate lyase fromErwinia carotovora based on electrostatic interaction
Endo-pectate lyase (PATE) fromErwinia carotovora was selectively cosedimented with extracellularly produced lipopolysaccharide-lipid complex (LPSLC) through dialysis of the cell free culture broth. The selective ...
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Article
Amplification immunoassay for the determination of Hepatitis B surface antigen
A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipo-polysacchari...
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Article
Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...
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Article
A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode
A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a d...
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Chapter
Microbial Sensors and Microbiosensors
A novel BOD sensor with a new principle employing a luminous bacterium, Photobacterium phosphoreum, was developed. The increased intensity of luminescence resulting from the cellular assimilation of organic compo...
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Article
Research and development of biosensors in Japan
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Article
Influence of cationic antibiotics on phase behavior of rough-form lipopolysaccharide
The rough-form lipopolysaccharide (LPS) interacted with cationic antibiotic polymyxin B and gramicidin S in solution, and showed altered thermotropic phase behavior and viscoelasticity. The phase behavior was ...
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Article
Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria
The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...
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Article
Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems
Enzyme-catalyzed hydrolyzations of starch by α-amylase have been studied in various two-phase systems, consisting of water and a water-immiscible organic solvent. The hydrolytic conversion of soluble starch to...
