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Article
Nucleus-translocated mitochondrial cytochrome c liberates nucleophosmin-sequestered ARF tumor suppressor by changing nucleolar liquid–liquid phase separation
The regular functioning of the nucleolus and nucleus-mitochondria crosstalk are considered unrelated processes, yet cytochrome c (Cc) migrates to the nucleus and even the nucleolus under stress conditions. Nucleo...
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Article
Open AccessDltC acts as an interaction hub for AcpS, DltA and DltB in the teichoic acid d-alanylation pathway of Lactiplantibacillus plantarum
Teichoic acids (TA) are crucial for the homeostasis of the bacterial cell wall as well as their developmental behavior and interplay with the environment. TA can be decorated by different modifications, modula...
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Protocol
In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies
The synthesis is an efficient strategy to produce in large scale protein samples for structural investigations. In vitro synthesis allows for significant reduction of production time, simplification of purif...
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Article
Open AccessStructural features of the interaction of MapZ with FtsZ and membranes in Streptococcus pneumoniae
MapZ localizes at midcell and acts as a molecular beacon for the positioning of the cell division machinery in the bacterium Streptococcus pneumoniae. MapZ contains a single transmembrane helix that separates the...
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Article
Open AccessInteraction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly
Transport of lipopolysaccharides (LPS) to the surface of the outer membrane is essential for viability of Gram-negative bacteria. Periplasmic LptC and LptA proteins of the LPS transport system (Lpt) are respon...
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Article
Open AccessSlow conformational exchange and overall rocking motion in ubiquitin protein crystals
Proteins perform their functions in solution but their structures are most frequently studied inside crystals. Here we probe how the crystal packing alters microsecond dynamics, using solid-state NMR measureme...
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Article
Open AccessObserving the overall rocking motion of a protein in a crystal
The large majority of three-dimensional structures of biological macromolecules have been determined by X-ray diffraction of crystalline samples. High-resolution structure determination crucially depends on th...
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Article
An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein
A strategy for the introduction of (1H,13C-methyl)-alanine into perdeuterated proteins is described. Specific protonation of alanine methyl groups to a level of 95% can be achieved by overexpressing proteins in M...
