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Protocol
Measuring Transcription Dynamics of Individual Genes Inside Living Cells
Transcription is a highly dynamic process, which, for many genes, occurs in stochastic bursts. Studying what regulates these stochastic bursts requires visualization and quantification of transcription dynamic...
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Article
Open AccessDynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live...
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Article
Correction: Corrigendum: Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA
Nature 535, 566–569 (2016); doi:10.1038/nature18643 The Acknowledgements of this Letter should have included the following sentence: “The research leading to these results received funding from LASERLAB-EUROPE...
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Protocol
Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics
DNA metabolism and DNA compaction in vivo involve frequent interactions of remote DNA segments, mediated by proteins. In order to gain insight into such interactions, quadruple-trap optical tweezers have been ...
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Protocol
Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments
Optical manipulation techniques provide researchers the powerful ability to directly move, probe and interrogate molecular complexes. Quadruple optical trap** is an emerging method for optical manipulation a...
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Article
Sliding sleeves of XRCC4–XLF bridge DNA and connect fragments of broken DNA
A combination of single-molecule techniques shows that the repair proteins XRCC4 and XLF form heteromeric mobile sleeve-like complexes that can bridge and hold together fragments of broken DNA.
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Article
Open AccessDirect quantitative detection of Doc2b-induced hemifusion in optically trapped membranes
Ca2+-sensor proteins control the secretion of many neuroendocrine substances. Calcium-secretion coupling may involve several mechanisms. First, Ca2+-dependent association of their tandem C2 domains with phosphati...
