Transgenic Crops of the World
Essential Protocols
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Genetic Engineering of Saccharum
Over the last two decades, substantial progress has been made in the genetic engineering of sugarcane (Saccharum spp.) through improvements in tissue culture procedures, allowing a higher efficiency of generating...
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Article
Influence of surfactants on growth and regeneration from mature internodal stem segments of sweet orange (Citrus sinensis) cv. Hamlin
The development of a reliable shoot regeneration system for mature tissue of citrus is of major importance to accelerate the evaluation of commercial traits. Three non-ionic surfactants were evaluated independ...
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Article
Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)
A reliable Agrobacterium-mediated transformation and shoot regeneration protocol was developed for breeding lines of commercially important western-shipper cantaloupe and honeydew melons, ‘F39’ and ‘150’, respect...
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Article
Erratum to: Genetic engineering of radish: current achievements and future goals
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Article
Genetic engineering of radish: current achievements and future goals
Radish is a major root crop grown in the Far East and is especially important to some low-income countries where it is consumed on a daily basis. Developments in gene technology systems have helped to accelera...
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Article
Sugarcane DIRIGENT and O-METHYLTRANSFERASE promoters confer stem-regulated gene expression in diverse monocots
Transcription profiling analysis identified Saccharum hybrid DIRIGENT (SHDIR16) and Ο-METHYLTRANSFERASE (SHOMT), putative defense and fiber biosynthesis-related genes that are highly expressed in the stem of suga...
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Protocol
Lettuce (Lactuca sativa L.)
Lettuce is a globally important leafy vegetable with the United States being the largest world producers. The crop is susceptible to a number of viruses that are aphid transmitted and also highly vulnerable to...
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Article
Modification of plant architecture through the expression of GA 2-oxidase under the control of an estrogen inducible promoter in Arabidopsis thaliana L
The gibberellin (GA) 2-oxidase (PcGA2ox1) from bean catalyses the 2β-hydroxylation of some precursor and bioactive GAs resulting in their inactivation. We have expressed PcGA2ox1 under the control of the estrogen...
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Optimized Shoot Regeneration System for the Commercial Korean Radish ‘** Ju Dae Pyong’
We have investigated the usefulness of hypocotyl (cultured on N1B2 medium) and cotyledon explants (on CR medium: Murashige and Skoog (MS) medium, 3% sucrose, 20 µm benzylaminopurine, pH 5.8) for the regenerati...
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Protocol
Production of Transgenic Crops by the Floral-Dip Method
The application of floral dip** toward the production of transformed plants has been rather limited. However, this procedure has enabled the successful production of transformed Medicago truncatula plants (a mo...
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Article
Expression of an antisense GIGANTEA (GI) gene fragment in transgenic radish causes delayed bolting and flowering
A late-flowering transgenic radish has been produced by the expression of an antisense GIGANTEA (GI) gene fragment using a floral-dip method. Twenty-five plants were dipped into a suspension of Agrobacterium c...
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Article
Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis
Transgenic plants of Arabidopsis thaliana (L.) Heynh. (ecotype Columbia) expressing the antisense AtMECT gene, encoding 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, were generated to elucidate the p...
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Article
Transgenic radish (Raphanus sativus L. longipinnatus Bailey) by floral-dip method – plant development and surfactant are important in optimizing transformation efficiency
Transgenic radish (Raphanus sativus L. longipinnatus Bailey) plants were produced from the progeny of plants which were dipped into a suspension of Agrobacterium carrying both the β-glucuronidase (gusA) gene and ...
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Protocol
Leaf Disk Transformation
Reliable and efficient methods of transferring cloned genes into plants are essential for engineering crops with desired traits. The Gram-negative soil bacteria, Agrobacterium tumefaciens and A. rhizogenes, are n...
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Protocol
NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
The stable introduction of genes into plants through genetic engineering normally necessitates the use of a selectable marker, especially when the transformation frequency is low (e.g., 1.0 × 10−3 to 10−6). Marke...
