48 Result(s)

Article

Contributory presentations/posters

N. Manoj, V. R. Srinivas, A. Surolia, M. Vijayan, K. Suguna… in Journal of Biosciences (1999)

Article

Conformational Difference between Ricin and Ricin Agglutinin in Solution and Crystal

N. N. Brandt, A. Yu. Chikishev, A. I. Sotnikov… in Doklady Biochemistry and Biophysics (2001)

Article

Intracellular Antibodies Do not Affect Transport of Protein Toxins

M. M. Moisenovich, I. I. Agapov, S. G. Egorova… in Doklady Biochemistry and Biophysics (2001)

Article

Topology of the polypeptide chain in the complex of agglutinin from castor bean seeds with β-D-galactose in the crystalline state

The three-dimensional structure of the complex of agglutinin from Ricinus communis with β-D-galactose was established and refined at 2.5 Å resolution by X-ray structure analysis. Biocrystals were obtained using d...

N. V. Konareva, A. G. Gabdulkhakov, S. Eschenburg, S. Stoeva… in Crystallography Reports (2001)

Article

Ricin and Viscumin Bind to Different Sites of the Cell Membrane

M. M. Moisenovich, I. A. Demina, I. I. Agapov… in Doklady Biological Sciences (2002)

Article

Role of the Interchain Interaction Domain of Chain A in Viscumin Cytotoxicity

The sequence coding for the viscumin (mistletoe lectin I, MLI) A-chain (MLA) was cloned from Viscum album genomic DNA with the use of synthetic primers. This yielded three recombinant (r) MLA variants differing i...

A. G. Tonevitskii, I. I. Agapov, N. V. Malyuchenko, M. M. Moisenovich… in Molecular Biology (2002)

Article

A New Antigenic Epitope Appears in the Catalytic Subunit of Viscumin during Intracellular Transport

The plant toxin viscumin (60 kD) consists of B- (“binding”) and A- (“active”) subunits joined by a disulfide bond. The B-subunit is a lectin interacting with galactose-containing glycolipids and glycoproteins ...

A. G. Tonevitsky, I. I. Agapov, M. M. Moisenovich… in Biochemistry (Moscow) (2003)

Article

An Epitope of the Viscumin Catalytic Subunit Exposed on Its Interaction with Lipid Bilayer

It was previously shown that the catalytic subunit of the plant toxin viscumin induces aggregation of small unilamellar liposomes and this process is inhibited by the mab_TA7 monoclonal antibody produced to th...

V. S. Pashkov, I. I. Agapov, T. A. Balashova… in Russian Journal of Bioorganic Chemistry (2003)

Article

Monovalent and Multivalent Binding of Streptavidin to Biotinylated Gramicidin Affects the Kinetic Properties of the Ion Channel

Biotin–avidin (or streptavidin) high affinity binding has been widely applied as a universal tool for basic research as well as diagnostic and therapeutic purposes. Here we studied the interaction of streptavi...

Y. N. Antonenko, T. I. Rokitskaya, E. A. Kotova, I. I. Agapov… in Biochemistry (Moscow) (2004)

Article

Cloning and Expression of Catalytic Subunit of MLIII, the Ribosome-Inactivating Protein from Viscum album

We have cloned the gene encoding a precursor of mistletoe (Viscum album) toxin MLIII. Analyses of nucleotide and deduced amino acid sequences of this gene revealed significant differences between MLI and MLIII pr...

A. G. Tonevitsky, I. I. Agapov, I. B. Pevzner, N. V. Malyuchenko… in Biochemistry (Moscow) (2004)

Article

Humoral immune response to recombinant viral NS3 protein in patients with hepatitis C

Full-length recombinant NS3 protein was used in a test system for detection of specific antibodies in the sera from patients with hepatitis C. Possible antigenic determinants in NS structure were predicted. It...

E. A. Gudim, I. I. Agapov, I. S. Komolov… in Bulletin of Experimental Biology and Medic… (2005)

Article

Cloning and expression of mistletoe lectin III B-subunit

Aqueous extracts of mistletoe (Viscum album L.) contain toxic proteins (lectins) MLI (viscumin), MLII, and MLIII. We previously cloned the gene encoding MLIII precursor. In the present study, a gene fragment enco...

I. B. Pevzner, I. I. Agapov, U. Pfueller, K. Pfueller… in Biochemistry (Moscow) (2005)

Article

A study of E. coli and T. maritima ribosomes by atomic force microscopy

Whole 70S ribosomes and 50S and 30S ribosomal subunits of E. coli and T. maritima were studied by atomic force microscopy. Adsorption of the ribosomal subunits on a substrate revealed considerable heterogeneity o...

N. V. Malyuchenko, E. A. Tonevitsky, I. I. Agapov, I. B. Pevzner, V. A. Bykov… in Biophysics (2006)

Article

Molecular genetic aspects of individual differences in pain sensitivity and thermoregulation

The molecular genetic aspects of individual differences in thermoregulation and pain sensitivity that may be used to evaluate and predict the physical state of humans under contrast climate conditions, as well...

E. V. Rostovtseva, E. A. Bondareva, I. I. Agapov in Human Physiology (2009)

Article

Three-dimensional scaffold made from recombinant spider silk protein for tissue engineering

I. I. Agapov, O. L. Pustovalova, M. M. Moisenovich… in Doklady Biochemistry and Biophysics (2009)

Article

A new method for quantitative estimation of the virus particles number

The virus particles of live mumps virus vaccine widely used for vaccination in Russia have been detected and visualized by the atomic force microscopy. For quantitative estimation of the number of observed vir...

A. S. Shebanova, M. N. Savvateev… in Biochemistry (Moscow) Supplement Series B:… (2009)

Article

Biodegradable matrices from regenerated silk of Bombix mori

I. I. Agapov, M. M. Moisenovich, T. V. Vasilyeva… in Doklady Biochemistry and Biophysics (2010)

Article

Biocompatible materials from regenerated silk for tissue engineering and medicinal therapy

The present review is devoted to the application of biomaterials from regenerated silk for designing tissue-engineered constructs—the basis for hybrid organs and tissues. Fibroin, the main structural protein o...

A. S. Kon’kov, O. L. Pustovalova, I. I. Agapov in Applied Biochemistry and Microbiology (2010)

Article

Study of lamellae of a recombinant spider-web protein by atomic force microscopy

Lamellae formed on mica by protein 1F9, a recombinant analog of spider web protein, have been studied by atomic force microscopy. It is shown that the molecules of 1F9 dissolved in strong solvents are capable ...

D. V. Bagrov, V. V. Prokhorov, D. V. Klinov, I. I. Agapov, I. V. Yaminskii… in Biophysics (2011)

Article

Biocomposite scaffolds containing regenerated silk fibroin and nanohydroxyapatite for bone tissue regeneration

I. I. Agapov, M. M. Moisenovich, T. V. Druzhinina… in Doklady Biochemistry and Biophysics (2011)