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  1. No Access

    Protocol

    Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies

    Peptide-major histocompatibility complex class II (pMHCII) multimers have emerged as a convenient and powerful tool for characterization of CD4 T cell immune responses in a large variety of human diseases. Pep...

    I-Ting Chow, William W. Kwok in T-Helper Cells (2021)

  2. Article

    Open Access

    Ontogeny of different subsets of yellow fever virus-specific circulatory CXCR5+ CD4+ T cells after yellow fever vaccination

    Monitoring the frequency of circulatory CXCR5+ (cCXCR5+) CD4+ T cells in periphery blood provides a potential biomarker to draw inferences about T follicular helper (TFH) activity within germinal center. However,...

    Quinn DeGottardi, Theresa J. Gates, Junbao Yang, Eddie A. James… in Scientific Reports (2020)

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  3. Article

    Open Access

    Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

    MHC tetramers are an essential tool for characterizing antigen-specific CD4+ T cells. However, their ex vivo analysis is limited by the large sample requirements. Here we demonstrate a combinatorial staining appr...

    Hannes Uchtenhagen, Cliff Rims, Gabriele Blahnik, I-Ting Chow… in Nature Communications (2016)

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  4. No Access

    Article

    Disulphide-isomerase-enabled shedding of tumour-associated NKG2D ligands

    A number of advanced tumours appear to evade immune recognition by natural killer cells by shedding the soluble major histocompatibility complex class I-related ligand MICA, which inactivates the NKG2D recepto...

    Brett K. Kaiser, Daesong Yim, I-Ting Chow, Segundo Gonzalez, Zhenpeng Dai… in Nature (2007)