-
Article
Oxidation and reduction analysis of therapeutic recombinant human interleukin-15 by HPLC and LC-MS
Being an important immune stimulant of T lymphocytes and NK cells, the recombinant human interleukin-15 (rhIL-15) has been extensively researched in tumor immunotherapy or as a vaccine adjuvant. However, the r...
-
Article
Open AccessA diagnostic model of autoimmune hepatitis in unknown liver injury based on noninvasive clinical data
All the diagnostic criteria of autoimmune hepatitis (AIH) include histopathology. However, some patients may delay getting this examination due to concerns about the risks of liver biopsy. Therefore, we aimed ...
-
Article
A homodimeric IL-15 superagonist F4RLI with easy preparation, improved half-life, and potent antitumor activities
Interleukin-15 (IL-15) is a promising candidate for cancer immunotherapy due to its potent immune-activating effects. There are several IL-15 molecules currently in clinical trials but facing shortages of poo...
-
Article
Open AccessSHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli
In previous work, we developed an E. coli extracellular secretion platform XTHHly based on the hemolysin A secretion system. It can produce bioactive peptides with simple purification procedures. However, the wid...
-
Article
Anti-bacterial monoclonal antibodies: next generation therapy against superbugs
Prior to the nineteenth century, infectious disease was one of the leading causes of death. Human life expectancy has roughly doubled over the past century as a result of the development of antibiotics and vac...
-
Article
Kinetics study of the natural split Npu DnaE intein in the generation of bispecific IgG antibodies
Rapid and efficient bispecific antibody (BsAb) production for industrial applications is still facing many challenges. We reported a technology platform for generating bispecific IgG antibodies, “Bispecific An...
-
Article
Open AccessA hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli
Although Escherichia coli has been widely used for the expression of exogenous proteins, the secretory expression in this system is still a big obstacle. As one of the most important secretion pathways, hemolysin...
-
Article
Single domain antibody-based vectors in the delivery of biologics across the blood–brain barrier: a review
Biologics are a promising and effective method for the treatment of central nervous system (CNS) diseases. The blood–brain barrier (BBB) is a natural barrier for the delivery of biologics into the brain, which...
-
Article
Open AccessA novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer
Prolactin receptor (PRLR) is highly expressed in a subset of human breast cancer and prostate cancer, which makes it a potential target for cancer treatment. In clinical trials, the blockade of PRLR was shown ...
-
Article
Antibody glycosylation: impact on antibody drug characteristics and quality control
Glycosylation is a common post-translational modification that occurs during the production of antibodies. Glycans attached to antibodies play an important role in the pharmacokinetics, efficacy, and safety of...
-
Article
Open AccessTheoretical screening of novel electrode materials for lithium–ion batteries from industrial polymers
Organic polymers have the potential to be electrode materials for lithium–ion batteries due to their lower solubility, lower self-discharge rates, high mechanical strength, greater flexibility, superior therma...
-
Article
Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production
To inspect the feasibility of recombinant stable HEK293 cell lines development for biopharmaceuticals production using CRISPR/Cas9-mediated site-specific integration.
-
Article
A general platform for efficient extracellular expression and purification of Fab from Escherichia coli
Antigen-binding fragments (Fabs) are an important part of monoclonal antibody (mAb) therapeutics and can be cost-effectively produced using an Escherichia coli (E. coli) expression system. However, Fabs tend to f...
-
Article
Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35
Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration strategy, development of a high-expressing and stable ...
-
Article
Open AccessEfficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system
Many methods have been developed to produce bispecific antibodies (BsAbs) for industrial application. However, huge challenges still remain in synthesizing whole length BsAbs, including their assembly, stabili...
-
Article
Purification of inclusion bodies using PEG precipitation under denaturing conditions to produce recombinant therapeutic proteins from Escherichia coli
It has been documented that the purification of inclusion bodies from Escherichia coli by size exclusion chromatography (SEC) may benefit subsequent refolding and recovery of recombinant proteins. However, loadin...
-
Article
A novel self-cleavable tag Zbasic–∆I-CM and its application in the soluble expression of recombinant human interleukin-15 in Escherichia coli
Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic–intein has been ...
-
Article
The chemokine CXCL9 exacerbates chemotherapy-induced acute intestinal damage through inhibition of mucosal restitution
Acute intestinal damage induced by chemotherapeutic agent is often a dose-limiting factor in clinical cancer therapy. The aim of this study was to investigate the effect of chemokine CXCL9 on the intestinal da...
-
Article
Interleukin-1 receptor antagonist attenuates cyclophosphamide-induced mucositis in a murine model
Cyclophosphamide is a cytotoxic chemotherapy drug that causes severe damages to hematopoietic and gastrointestinal systems. The aim of this study is to evaluate the protective effects of recombinant human inte...
-
Article
Expression, Purification, and Characterization of a Novel Soluble Form of Human Delta-like-1
The notch signaling pathway plays an important role in inhibiting cell differentiation and enhancing the repopulation capability of hematopoietic stem/progenitor cells. In this study, we developed rhDSL, a nov...
