1,376 Result(s)
within
Chemistry
Biotechnology
Hu **ao
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Article
AOS — An anionic surfactant system: Its manufacture, composition, properties, and potential application
Several sulfonation parameters, believed to be critical to the manufacture of good quality a-olefin sulfonate (AOS), are related to product color and conversion. The interfacial properties for single carbon nu...
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Chapter
The Effects of Polyoxyethylene Chain Length Distribution on the Interface Properties of Polyoxyethylenated N-Dodecyl Alcohols
Assuming ideal mixing behavior, the effects of different Gaussian distribution in polyoxyethylene (POE) chain length of surfactants prepared from mono-polyoxyethylene glycol n-dodecyl alcohols on the thermodyn...
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Article
A mechanistic approach to the thermal degradation of α-olefin sulfonates
Determination of the thermal stability of α-olefin sulfonates (AOS) via the conventional activation energy approach was found to be impractical because of the difficulties arising from the fact that α-olefin s...
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Article
Effect of water hardness ions on the solution properties of an anionic surfactant
The solution characteristics of the system linear alkylbenzene sulfonate (LAS)/Ca++/builder were studied using a dynamic surface tension technique. The results showed that the rate of CA++/LAS interaction is slow...
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Chapter and Conference Paper
Stoichiometric Considerations of Mammalian Cell Metabolism in Bioreactors
For mammalian cells in culture the primary energy sources include glucose and glutamine. Under normal culture conditions, both glucose and glutamine are essential for cell growth. At a high glucose concentrati...
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Chapter
A Correlation between Photocycle and Photoelectric Response of Bacteriorhodopsin Monomers
The bacteriorhodopsin (BR) molecules in the purple membrane (PM) are organized into a two-dimensional hexagonal lattice of trimers which are surrounded by about 30 lipid molecules. The protein-protein and prot...
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Article
Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study
Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. th...
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Article
Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation
Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied sho...
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Article
A two-compartment cell entrapment bioreactor with three different holding times for cells, high and low molecular weight compounds
A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collage...
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Article
Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining
Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain ...
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Article
A cartridge oxygenator for mammalian cell culture in a stirred tank bioreactor
Tubing made of membrane with high oxygen permeability is often used in supplying oxygen to animal cell culture bioreactors. We have fabricated the tubing into a cartridge configuration. Such an arrangement all...
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Article
Virus production in microsphere-induced aggregate culture of animal cells
Green monkey kidney cells (Vero) were cultivated on microspheres with a median diameter of 23 μm. Aggregates were formed two days after inoculation and eventually reached a few hundred micrometers in diameter....
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Article
Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels
Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tensio...
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Article
Engineering perspectives in tissue engineering
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Article
Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver
A static culture model employing cylindrical collagen-hepatocyte gels is reported for large scale testing of conditions relevant to the three compartment hollow fiber bioartificial liver. High density hepatocy...
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Chapter and Conference Paper
A Model for Embryo Development in Dicotyledonous Plants
With the employment of genetic engineering techniques to alter the genetic makeup of plants, the ability to regenerate whole plants through tissue culture becomes crucial. Plantlet micropropagation based on sh...
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Chapter and Conference Paper
Kinetics of Hybridoma Cell Growth in Continuous Culture
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Article
Inductively coupled plasma mass spectrometry with an enlarged sampling orifice and offset Ion Lens. I. Ion trajectories and detector performance
A new inductively coupled plasma mass spectrometer (ICP-MS) with four stages of differential pum** is described. The relatively large sampling orifice (1.31-mm dia.) improves signals for metal ions and resis...
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Article
Inductively coupled plasma mass spectrometry with an enlarged sampling orifice and offset ion lens. II. Polyatomic Ion interferences and matrix effects
A new inductively coupled plasma mass spectrometer with an enlarged sampling orifice (1.31-mm dia.) and an offset ion lens yields very low levels of many troublesome polyatomic ions such as ArO+, ArN+, Ar2 ...
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Article
A sensitive measurement of oxygen uptake rate using a optochemical sensor
The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we...
