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Protocol
Development of a Bioartificial Liver Device
Liver disease continues to be a challenge clinically, with 30,000 patients dying each year from liver failure (1). Although liver transplantation can successfully treat many patients undergoing liver failure, the...
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Article
Development of a bioartificial liver employing xenogeneic hepatocytes
Liver failure is a major cause of mortality. A bioartificial liver (BAL) employing isolated hepatocytes can potentially provide temporary support for liver failure patients. We have developed a bioartificial l...
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Article
Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids
Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. U...
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Article
Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel
The potential use of porcine hepatocytes in a bioartificial liver device requires large quantities of viable and highly active cells. To facilitate the scaling up of the system, liver specific activities of he...
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Article
A sensitive measurement of oxygen uptake rate using a optochemical sensor
The measurement of the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we...
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Article
Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver
A static culture model employing cylindrical collagen-hepatocyte gels is reported for large scale testing of conditions relevant to the three compartment hollow fiber bioartificial liver. High density hepatocy...
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Article
Microelectrode measurement of oxygen tensions in collagen-hepatocyte gels
Microelectrodes have been used for the measurement of oxygen tension in various biological systems.(Silver, 1987) Although not previously reported, microelectrodes allow the direct measurement of oxygen tensio...
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Chapter
Cultivation of Hepatocytes in a New Entrapment Reactor: a Potential Bioartificial Liver
Liver failure is a major cause of mortality.1 Most patients admitted to an intensive care unit in liver failure do not survive. Currently, the only available treatment for refractory liver failure is hepatic tran...
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Article
AMINO ACIDS IN INFANTS WITH NON-CORRECTED EXTRA-HEPATIC BILIARY ATRESIA (EBA)
Feeding formulations which are enriched in branch chain amino acids (BCAA) and depleted in aromatic amino acids (AAA) and methionine (Met) are used in therapy of adults with hepatic encephalopathy (HE). These ...
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Chapter
Nutritional/Metabolic Support: Parenteral and Enteral
The literature is replete with studies and reviews analyzing the effects of nutritional support on patients with various stages of malignancy and undergoing various forms of treatment. Much uncertainty and con...
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Article
Arterial embolization for traumatic hemobilia with hepato-portal fistula
A case of blunt liver trauma complicated by delayed upper gastrointestinal bleeding, probably hemobilia, was successfully treated by intra-arterial embolization. Laparotomy with liver resection or hepatic arte...
