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Protocol
Light Response Curves in Land Plants
Light is the driving force for photosynthesis. Two techniques are commonly employed to help characterize the relationship between the light environment and photosynthesis in plants.
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Protocol
Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of Rice
Immunolocalization analysis is a principal tool to study protein expression and subcellular distribution in plant cells or tissues. In this chapter, we present the method of the preparation of lightly fixed fr...
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Article
Open AccessTransgenic maize phosphoenolpyruvate carboxylase alters leaf–atmosphere CO2 and 13CO2 exchanges in Oryza sativa
The engineering process of C4 photosynthesis into C3 plants requires an increased activity of phosphoenolpyruvate carboxylase (PEPC) in the cytosol of leaf mesophyll cells. The literature varies on the physiologi...
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Chapter
Climate-Resilient Future Crop: Development of C4 Rice
Rice is the most important crop in the world. It is a staple food for more than half of the human population and a primary food source for the world’s poorest people. Asia currently accounts for 90% of global ...
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Article
Open AccessCandidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
All grass leaves are strap-shaped with a series of parallel veins running from base to tip, but the distance between each pair of veins, and the cell-types that develop between them, differs depending on wheth...
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Article
CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
CRISPR-Cas9/Cpf1 system with its unique gene targeting efficiency, could be an important tool for functional study of early developmental genes through the generation of successful knockout pla...
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Article
High-level β1-integrin expression in a subpopulation of highly tumorigenic oral cancer cells
The β1 integrin (CD29) is a putative marker for cancerous epithelial stem cells. Cancer stem cells are essential to drive tumor growth, recurrence, and metastasis. We investigated the role of β1-integrin expre...
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Article
Isolating stromal stem cells from periodontal granulation tissues
Stem cell therapy is a promising area in regenerative medicine. Periodontal granulation tissues are often discarded during conventional surgery. If stromal stem cells can be isolated from these tissues, they c...
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Chapter and Conference Paper
An Emotional Designed Based Hybrid MCDM Framework for the Next Generation Embedded System Configurations
Emotions play a critical role in human’s ability to understanding and learning about the world and new things. Furthermore, the concept of user-centered system design (UCSD), which emphasizes the importance of...
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Article
Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeis guineensis: evidence for conserved gene functions in early development
With the aim of understanding the molecular mechanisms underlying somatic embryogenesis (SE) in oil palm, we examined transcriptome changes that occur when embryogenic suspension cells are initiated to develop...