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Article
Open AccessDiscovery and rational engineering of PET hydrolase with both mesophilic and thermophilic PET hydrolase properties
Excessive polyethylene terephthalate (PET) waste causes a variety of problems. Extensive research focused on the development of superior PET hydrolases for PET biorecycling has been conducted. However, templat...
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Article
Open AccessEnhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Malate dehydrogenase (MDH) is one of the key enzymes for SA...
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Article
Open AccessReply to “Conformational fitting of a flexible oligomeric substrate does not explain the enzymatic PET degradation”
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Article
Crystal Structure of a Novel Type Isomerase of Enoyl-CoA Hydratase/Isomerase Family Protein from Cupriavidus necator H16
Although enoyl-CoA hydratase/isomerase superfamily proteins are functionally diverse and extremely abundant in microbial and higher organism’s genome, they still have been elusively annotated. The genome of Cupri...
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Article
Open AccessIn silico-designed lignin peroxidase from Phanerochaete chrysosporium shows enhanced acid stability for depolymerization of lignin
The lignin peroxidase isozyme H8 from the white-rot fungus Phanerochaete chrysosporium (LiPH8) demonstrates a high redox potential and can efficiently catalyze the oxidation of veratryl alcohol, as well as the de...
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Article
Open AccessStructural insight into molecular mechanism of poly(ethylene terephthalate) degradation
Plastics, including poly(ethylene terephthalate) (PET), possess many desirable characteristics and thus are widely used in daily life. However, non-biodegradability, once thought to be an advantage offered by ...
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Article
Open AccessStructural basis for a novel type of cytokinin-activating protein
The Lonely Guy (LOG) protein has been identified as a crucial enzyme involved in the production of cytokinins, which are important phytohormones, in plants and plant-interacting organisms. However, C. glutamicum ...
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Article
Open AccessStructural basis for cytokinin production by LOG from Corynebacterium glutamicum
“Lonely guy” (LOG) has been identified as a cytokinin-producing enzyme in plants and plant-interacting fungi. The gene product of Cg2612 from the soil-dwelling bacterium Corynebacterium glutamicum was annotated a...
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Article
Open AccessCRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
Genome editing is crucial for genetic engineering of organisms for improved traits, particularly in microalgae due to the urgent necessity for the next generation biofuel production. The most advanced CRISPR/C...
