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Article
Decrease in stomach contents in the Antarctic minke whale (Balaenoptera bonaerensis) in the Southern Ocean
The Antarctic minke whale (Balaenoptera bonaerensis) is one of the major krill predators in Antarctic waters. A reported decline in energy storage over almost two decades indicates that food availability for the ...
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Article
The Co-Delivery of Oxaliplatin Abrogates the Immunogenic Response to PEGylated siRNA-Lipoplex
In vivo application of siRNA/PEGylated cationic liposome complex (lipoplex) is impeded by two main obstacles: cytokine responses and anti-PEG IgM responses to PEGylated siRNA-lipoplex. Here, we...
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Protocol
Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery
For the systemic application of nucleic acids such as plasmid DNA and small interfering RNA, safe and efficient carriers that overcome the poor pharmacokinetic properties of nucleic acids are required. A catio...
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Article
Habitat and prey selection of common minke, sei, and Bryde’s whales in mesoscale during summer in the subarctic and transition regions of the western North Pacific
This study represents the first quantitative analysis of the characteristics of the distribution areas and stomach contents of common minke whale Balaenoptera acutorostrata, sei whale B. borealis, and Bryde’s wha...
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Article
Improved intratumoral delivery of PEG-coated siRNA-lipoplexes by combination with metronomic S-1 dosing in a murine solid tumor model
Efficient systemic siRNA delivery to cells in the target tissue is a current critical challenge in the drug delivery field. Several studies have demonstrated that nanoparticles such as polyethylene glycol (PEG...
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Article
Argonaute2 is a potential target for siRNA-based cancer therapy for HT1080 human fibrosarcoma
Small interfering RNAs (siRNAs) are small RNA molecules that have a potent, sequence-specific gene silencing effect and therefore show promise for therapeutic use as molecular-targeted drugs for the treatment ...
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Article
Targeting Anticancer Drugs to Tumor Vasculature Using Cationic Liposomes
Liposomal drug delivery systems improve the therapeutic index of chemotherapeutic agents, and the use of cationic liposomes to deliver anticancer drugs to solid tumors has recently been recognized as a promisi...
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Protocol
Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation
In the last two decades, cationic liposomes have been investigated as vehicles for nucleic acids [plasmid DNA (pDNA) and small interfering RNA (siRNA)] delivery in vitro and in vivo. The formation of cationic ...
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Article
Accelerated Blood Clearance Phenomenon Upon Repeated Injection of PEG-modified PLA-nanoparticles
We recently developed prostaglandin E1 (PGE1)-encapsulated nanoparticles, prepared with a poly(lactide) homopolymer (PLA, Mw = 17,500) and monomethoxy poly(ethyleneglycol)-PLA block copolymer (PEG-PLA) (NP-L20). ...
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Article
Effect of Co-administration of Tacrolimus on the Pharmacokinetics of Multiple Subcutaneous Administered Interferon-Alpha in Rats
Repeated administration of exogenous proteinic compounds triggers the production of specific antibodies. This reaction is limits not only pharmacokinetic studies in animal but also development of human or huma...
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Chapter
Unexpected Reactions by In Vivo Applications of PEGylated Liposomes
PEGylated liposome, a liposome coated with polyethylene glycol (PEG), is understood to be biologically inert and, therefore, a suitable vehicle for in vivo applications. The most successful example is the doxo...
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Article
Global Gene Expression Profiling in Cultured Cells Is Strongly Influenced by Treatment with siRNA–Cationic Liposome Complexes
The purpose of this study is to determine if the treatment with siRNA-lipoplexes significantly influences on global gene expression in the treated cells.
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Article
Possible source of the antarctic bottom water in the Prydz Bay Region
It has been inferred that the Prydz Bay region is one of the source regions of Antarctic Bottom Water (AABW) based on rather indirect evidence. In order to examine this inference, we investigate the hydrograph...
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Article
Quantitative Analysis of Correlation Between Number of Nuclear Plasmids and Gene Expression Activity After Transfection with Cationic Liposomes
Purpose. A quantitative understanding of the intracellular trafficking of plasmids delivered by nonviral vectors is essential for optimizing vector functions to increase their transfection efficiency. In this st...
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Article
Liposome Clearance
The clearance rate of liposomal drugs from the circulation is determined by the rate and extent of both drug release and uptake of liposomes by cells of the mononuclear phagocyte system (MPS). Intravenously in...
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Article
Plasma Factor Triggering Alternative Complement Pathway Activation by Liposomes
Several plasma components, such as complement (C) components, play a role in the clearance of liposomes from the circulation. The interactions between liposomes and the C system were investigated in this study...
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Article
Enhanced Hepatic Uptake of Liposomes Through Complement Activation Depending on the Size of Liposomes
The objective of this study was to differentiate the roles of opsonins and phagocytic cells in the size-dependent hepatic uptake of liposomes in the submicron region. The extent of opsonization decreased with ...
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Article
Kinetic analysis of AUC-dependent saturable clearance of liposomes: Mathematical description of AUC dependency
The objective of this study was to examine the AUCdependency of saturable hepatic clearance (CL h )of liposomes and to postulate a mathematical model to describe the characteris...
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Article
Distinction Between the Depletion of Opsonins and the Saturation of Uptake in the Dose-Dependent Hepatic Uptake of Liposomes
Opsonins play a role in the hepatic uptake of particles such as bacteria, lipid emulsion, and liposomes. The objective of this study was to distinguish between opsonin depletion and uptake saturation in the do...
