-
Article
Open AccessA Ca2+/CaM-regulated transcriptional switch modulates stomatal development in response to water deficit
Calcium (Ca2+) signals are decoded by the Ca2+-sensor protein calmodulin (CaM) and are transduced to Ca2+/CaM-binding transcription factors to directly regulate gene expression necessary for acclimation responses...
-
Chapter
Water Sensing in Plants
Water is a key factor in plant life. Therefore, reaching and holding water is a crucial part in plant survival. Plants sense water through a set of sensors which includes sensors for water activity (potential)...
-
Article
Open AccessA transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via ...
-
Article
Open AccessTranscriptomic analysis of Sorghum bicolor responding to combined heat and drought stress
Abiotic stresses which include drought and heat are amongst the main limiting factors for plant growth and crop productivity. In the field, these stress types are rarely presented individually and plants are o...
-
Article
Open AccessGenome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling
The Amoebozoa constitute one of the primary divisions of eukaryotes, encompassing taxa of both biomedical and evolutionary importance, yet its genomic diversity remains largely unsampled. Here we present an an...
-
Article
Calmodulin-binding transcription activator 1 mediates auxin signaling and responds to stresses in Arabidopsis
Auxin is a key plant hormone that regulates various aspects of plant development. However, the mechanisms integrating auxin growth effects with stress responses are not fully understood. In this study, we inve...
-
Chapter
Physiological Roles of Cyclic Nucleotide Gated Channels in Plants
Plant cyclic nucleotide gated channels (CNGCs) are diverse in their temporal and spatial expression patters, their subcellular membrane localization, their involvement in signaling cascades, and their physiolo...
-
Chapter
GABA and GHB Neurotransmitters in Plants and Animals
γ-Aminobutyric acid (GABA) is a four-carbon non-protein amino acid conserved from bacteria to plants and vertebrates. In the latter it is mainly known as a neurotransmitter. The enzymes that sy...
-
Article
The root-specific glutamate decarboxylase (GAD1) is essential for sustaining GABA levels in Arabidopsis
In plants, as in most eukaryotes, glutamate decarboxylase catalyses the synthesis of GABA. The Arabidopsis genome contains five glutamate decarboxylase genes and one of these genes (glutamate decarboxylase1; i.e.
-
Chapter
Calmodulin and Plant Responses to the Environment
Calcium ions (Ca2+) function as a widespread messenger in mediating responses of eukaryotes to environmental signals, often through Ca2+-modulated proteins like calmodulin (CaM) (for review see Snedden and Fromm ...
-
Article
A high-affinity calmodulin-binding site in a tobacco plasma-membrane channel protein coincides with a characteristic element of cyclic nucleotide-binding domains
Recently we isolated a cDNA encoding a tobacco plasma membrane calmodulin-binding channel protein (designated NtCBP4) with a putative cyclic nucleotide-binding domain. Here we analyzed in detail the interactio...
-
Chapter
Characterization of a Novel Family of Calmodulin-Binding Plasma Membrane Channel-Like Proteins
Calcium (Ca2+) is a ubiquitous second messenger in all eukaryotes (reviewed by Clapham, 1995; Bush, 1995; McAinsh and Hetherington, 1998). Ca2+ signals are transduced primarily by Ca2+ modulated proteins such as ...
-
Article
Two isoforms of glutamate decarboxylase in Arabidopsis are regulated by calcium/calmodulin and differ in organ distribution
The nucleotide sequences of cDNAs encoding two isoforms of Arabidopsis glutamate decarboxylase, designated GAD1 (57.1 kDa) and GAD2 (56.1 kDa) and sharing 82% identical amino acid sequences, were determined. T...
-
Article
Developmentally regulated organ-, tissue-, and cell-specific expression of calmodulin genes in common wheat
Recently, we reported on the characterization of the calmodulin (CaM) gene family in wheat [44]. We classified wheat CaM genes into four subfamilies (SFs) designated SF-1 to SF-4, each representing a series of...
-
Article
Characterization of the plant homologue of prohibitin, a gene associated with antiproliferative activity in mammalian cells
This report describes the cloning and characterization of a plant cDNA coding for a protein which shows high amino acid sequence similarity with prohibitin, whose gene is associated with antiproliferative acti...
-
Article
PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis
One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3...
-
Article
Cloning of plant cDNAs encoding calmodulin-binding proteins using35S-labeled recombinant calmodulin as a probe
Radiolabelled calmodulin has previously been used to screen cDNA expression libraries to isolate calmodulin-binding proteins. We have modified this technique for the isolation of plant calmodulin-binding prote...
-
Article
The tobacco transcription activator TGA1a binds to a sequence in the 5′ upstream region of a gene encoding a TGA1a-related protein
We have isolated and characterized a tobacco gene, designated G13, encoding a leucine zipper DNA-binding protein related to the transcription activator TGA1a. The G13 coding region is divided into eight exons ...
-
Article
Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts
A streptomycin resistant Nicotiana plastome mutant, X/str R6, was subjected to molecular analysis. In this mutant, a single nucleotide transition, C » T, in the chloroplast gene for ribosomal protein S12 alters c...
-
Article
A novel site for streptomycin resistance in the “530 loop” of chloroplast 16S ribosomal RNA
The chloroplast gene for 16S rRNA was cloned from two maternally inherited streptomycin-resistant mutants ofNicotiana differing in degree of resistance at the whole plant and isolated chloroplast level. A single-...
