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  1. No Access

    Protocol

    Organ Bioluminescence Imaging Under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation

    Determination of organ viability over a period of time is a key technology in the process of organ preservation. However, a robust methodology to address this issue has not been established. Luciferase-express...

    Yuhei Higashi, Jun Homma, Hidekazu Sekine in Bioluminescence (2022)

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    Protocol

    Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure

    This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of smal...

    Hidekazu Sekine, Teruo Okano in Cardiac Tissue Engineering (2022)

  3. No Access

    Article

    Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro

    The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the...

    Yuji Haraguchi, Tatsuya Shimizu, Tadashi Sasagawa, Hidekazu Sekine… in Nature Protocols (2012)