116 Result(s)
within
Guocheng Du
Life Sciences
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Article
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli
To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molec...
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Article
Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae
Saccharomyces cerevisiae, a common eukaryotic model microorganism that is generally recognized as safe, is widely employed for the heterologous expression of edible and therapeutic proteins. Promoters are crucial...
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Article
Construction of a plasmid-free Escherichia coli strain for lacto-N-neotetraose biosynthesis
Lacto-N-neotetraose (LNnT), with promising bioactive properties, is one of the most significant nonfucosylated human milk saccharides. Recently, its synthesis by microbial fermentation has attracted great attenti...
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Article
Construction of immobilized enzyme cascades for the biosynthesis of nucleotide sugars UDP-N-acetylglucosamine and UDP-glucuronic acid
UDP-sugars, as active forms of monosaccharides, play integral roles in glycosylation and biosynthesis of polysaccharides. Although enzymatic catalysis has achieved great process, the comparatively low producti...
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Article
Metabolic engineering of Bacillus subtilis for de novo synthesis of 6′-sialyllactose
6′-sialyllactose (6′-SL) is an important component of human milk oligosaccharides (HMOs) and has numerous infant health benefits. The construction of efficient and food-safe microbial cell factories to produce 6′
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Article
Optimization of linear plasmid expression system for protein production and secretion in Bacillus thuringiensis
Bacillus thuringiensis, a safe bacterium widely used in agriculture for the biocontrol of pests, has great potential for protein production. The linear plasmid expression system, bacterial orthogonal DNA replicat...
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Engineered Saccharomyces cerevisiae for de novo δ-tocotrienol biosynthesis
As a vitamin E isomer, δ-tocotrienol has attracted much attention owing to its rich biological activities for human health, especially anticancer activities. Microbial biosynthesis constitutes a promising stra...
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Engineering Bacillus subtilis for efficient production of 3′-sialyllactose
3′-Sialyllactose (3′-SL) promotes the proliferation of the bifidobacterial population and shapes gut microbiota. Thus, it can be used in the infant formula industry. In this study, an engineered Bacillus subtilis
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Article
Crude enzyme immobilization-based cell-free system for efficient N-acetylneuraminic acid biosynthesis aided by N-terminal coding sequence screening
N-acetylneuraminic acid (NeuAc) is an important nutrient that plays a key role in brain development in infants NeuAc is mainly produced by extraction from natural resources such as edible birds’s nests, crucian.....
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Article
Combinatorial metabolic engineering enables high yield production of α-arbutin from sucrose by biocatalysis
α-Arbutin has been widely used as a skin-whitening ingredient. Previously, we successfully produced α-arbutin via whole-cell biocatalysis and found that the conversion rate of sucrose to α-arbutin was low (~45...
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Article
Enhanced catalytic efficiency and substrate specificity of Streptomyces griseus trypsin by evolution-guided mutagenesis
Streptomyces griseus trypsin (SGT) is a bacteria-sourced trypsin that could be potentially applied to industrial insulin productions. However, SGT produced by microbial hosts displayed low catalytic efficiency an...
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Article
In silico cell factory design driven by comprehensive genome-scale metabolic models: development and challenges
Genome-scale metabolic models (GEMs) have been widely used to design cell factories in silico. However, initial flux balance analysis only considers stoichiometry and reaction direction constraints, so it cann...
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Revealing novel synergistic defense and acid tolerant performance of Escherichia coli in response to organic acid stimulation
Escherichia coli is an important producer of mono- and di-acids, such as D-lactic acid, itaconic acid, and succinic acid. However, E. coli has limited acid tolerance and requires neutralizers in large-scale ferme...
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Open AccessProfiling the role of microorganisms in quality improvement of the aged flue-cured tobacco
The aging process in the tobacco production, as in other food industries, is an important process for improving the quality of raw materials. In the spontaneous aging, the complex components in flue-cured toba...
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Metabolite-based cell sorting workflow for identifying microbes producing carbonyls in tobacco leaves
Carbonyl compounds represented by aldehydes and ketones make an important contribution to the flavor of tobacco. Since most carbonyl compounds are produced by microbes during tobacco fermentation, identifying ...
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Article
Food-grade expression of multicopper oxidase with improved capability in degrading biogenic amines
Biogenic amines (BAs) are potential amine hazards that are detected in fermented foods and alcoholic beverages. Excessive intake of BAs may lead to allergic symptoms such as difficulty in breathing, nausea, an...
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Article
Chitin deacetylase: from molecular structure to practical applications
Chitosan and chitooligosaccharides (COS), as derivatives of chitin through deacetylation reaction, have broad applications due to their good biodegradability, biocompatibility, and solubility. In addition, chi...
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Article
Enzymatic production of N-acetylneuraminic acid: advances and perspectives
N-Acetyl-d-neuraminic acid (NeuAc), a well-known and well-studied sialic acid, is found in cell surface glycolipids and glycoproteins, where it performs a variety of biological functions. The use of NeuAc as a nu...
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The challenges and prospects of Escherichia coli as an organic acid production host under acid stress
Organic acids have a wide range of applications and have attracted the attention of many industries, and their large-scale applications have led fermentation production to low-cost development. Among them, the...
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Bioaugmentation of Bacillus amyloliquefaciens–Bacillus kochii co-cultivation to improve sensory quality of flue-cured tobacco
Flue-cured tobacco (FCT) with irritating and undesirable flavor must be aged. However, the spontaneous aging usually takes a very long time for the low efficiency. Bioaugmentation with functional strains is a ...
