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Article
Open AccessThe ubiquitin-like modifier FAT10 interferes with SUMO activation
The covalent attachment of the cytokine-inducible ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) to hundreds of substrate proteins leads to their rapid degradation by the 26 S proteasome independ...
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Article
Open AccessAuthor Correction: The structure of the ubiquitin-like modifier FAT10 reveals an alternative targeting mechanism for proteasomal degradation
The original version of the Supplementary Information associated with this Article inadvertently omitted Supplementary Table 3. The HTML version of the Article has been updated to include a corrected version o...
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Article
Open AccessThe structure of the ubiquitin-like modifier FAT10 reveals an alternative targeting mechanism for proteasomal degradation
FAT10 is a ubiquitin-like modifier that directly targets proteins for proteasomal degradation. Here, we report the high-resolution structures of the two individual ubiquitin-like domains (UBD) of FAT10 that ar...
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Article
FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis
FAT10 is the only ubiquitin-like modifier that can target proteins for degradation by the proteasome in a ubiquitin-independent manner. The degradation of FAT10-linked proteins by the proteasome is strongly ac...
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Article
USE1 is a bispecific conjugating enzyme for ubiquitin and FAT10, which FAT10ylates itself in cis
The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction part...
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Protocol
Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR
The 26S proteasome is the executing protease of the ubiquitin-dependent degradation system. It consists of one or two 19S regulatory sub-complexes and one 20S proteolytic sub-complex (1). The 20S proteasome is a ...
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Protocol
Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma
Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable in...
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Chapter
Effects of the HIV-1 Protease Inhibitor Ritonavir on Proteasome Activity and Antigen Presentation
Ritonavir is a potent inhibitor of the protease encoded by the human immunodeficiency virus (HIV)-1 and is clinically applied to suppress HIV-1 replication in AIDS patients. When following up clinical hints po...
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Article
Structure and structure formation of the 20S proteasome
Eukaryotic 20S proteasomes are complex oligomeric proteins. The maturation process of the 14 different α- and β-subunits has to occur in a highly coordinate manner. In addition β-subunits are synthesized as pr...
