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Article
Androgen signaling is essential for development of prostate cancer initiated from prostatic basal cells
Emerging evidence has shown that both prostatic basal and luminal cells are able to initiate oncogenic transformation. However, despite the diversity of tumor-initiating cells, most prostate cancer cells expre...
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Article
Current understanding of hypospadias: relevance of animal models
Hypospadias occurs in approximately 1:200–1:300 newborn males, and is the second most common congenital abnormality in boys
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Article
Tissue Recombination Techniques for Mouse Embryonic Mammary Glands
This review gives detailed technical protocols for dissection of embryonic mammary rudiments and preparation of tissue recombinants composed of embryonic mouse mammary mesenchyme and epithelium. This experimen...
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Article
Androgen receptor is overexpressed in boys with severe hypospadias, and ZEB1 regulates androgen receptor expression in human foreskin cells
ZEB1 is overexpressed in patients with severe hypospadias. We examined the interaction between ZEB1 and the androgen receptor (AR) in vitro and the expression of AR in boys with hypospadias.
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Article
Formation of human prostate tissue from embryonic stem cells
Rodent models and immortalized or genetically modified cell lines are frequently used—but have limited utility—for studying human prostate development and maturation. Using rodent mesenchyme to establish recip...
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Chapter and Conference Paper
Development of the Penile Urethra
In humans hypospadias consists of an abnormal ventral urethral meatus, incomplete formation of the prepuce and penile curvature (Baskin et al., 1998). The cause of hypospadias is thought to be failure of formatio...
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Article
Expression of the androgen receptor and 5α-reductase type 2 in the develo** human fetal penis and urethra
Normal penile development is dependent on testosterone, its conversion via steroid 5α-reductase type 2 to dihydrotestosterone, and a functional androgen receptor (AR). The goal of this study was to investigat...
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Article
Urethral seam formation and hypospadias
Knowledge of the formation of the normal male urethra may elucidate the etiology of hypospadias. We describe urethral formation in the mouse, show the similarities and relevance to human urethral development,...
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Chapter and Conference Paper
Stromal Influences in Prostatic Carcinogenesis
It has been estimated that 184,500 new cases of prostate cancer (PRCA) will be diagnosed in the USA during 1998 and that 39,200 deaths will result from this disease. Thus, PRCA is the most commonly diagnosed m...
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Chapter
Transplantation and Tissue Recombination Techniques to Study Mammary Gland Biology
Because certain mutant transgenic mice die prematurely or are infertile, the study of the mammary gland is hampered. To circumvent this problem, mammary transplantation techniques can be used to “rescue” mamma...
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Chapter
Ultrastructural Smooth Muscle Ontogeny of the Rat Bladder
The transmission electron microscope characteristics of develo** rat bladder smooth muscle are described at 14 and 18 days of gestation, birth, and adulthood. Caveolae, microfilaments, and dense bodies incre...
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Chapter
Epithelial-Mesenchymal Interactions in the Bladder
The urinary bladder has two major functions: 1) storage and 2) socially acceptable efficient emptying.1 To achieve this end the bladder has evolved as a highly compliant muscular sac with a specialized epithelial...
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Article
The Role of Type IV Collagenases in Rat Bladder Development and Obstruction
The role of type IV collagenases during rat bladder development and in response to partial bladder outlet obstruction was evaluated. Gelatinase gel zymography was performed on develo** rat bladders (gestatio...
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Article
Role of mesenchymal-epithelial interactions in mammary gland development
The mammary gland is a hormone-target organ derived from epidermis and develops as a result of reciprocal mesenchymal-epithelial interactions. The induction of mammary differentiation from indifferent epiderma...
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Chapter
Role of Mesenchymal-Epithelial Interactions in Normal and Abnormal Development of Male Urogenital Glands
Malignant cells usually fail to express specialized functions of the tissue from which they arise and are frequently undifferentiated. The loss of specialized function in carcinoma cells has been attributed to...
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Chapter
Stromal regulation of epithelial function
Stromal influences upon epithelia are part of a continuum of cellular interactions that begins at fertilization and extends into adulthood. In parenchymal organs, the most thoroughly characterized interactions...
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Article
Serum-free culture of enriched mouse anterior and ventral prostatic epithelial cells in collagen gel
Sustained growth of mouse ventral and anterior prostatic epithelial cells embedded within collagen gel matrix was achieved in a serum-free medium composed of Dulbecco's modified Eagle's medium and Ham's F12 me...
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Chapter
Morphogenesis and Cytodifferentiation of Male Sex Accessory Epithelia: Involvement of the Mesenchyme and Neurotransmitters
Androgenic steroid hormones characteristically regulate the expression of specific genes within their target organs (Higgins and Parker, 1980; Cunha et al., 1987). Since the advent of recombinant DNA technology, ...
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Chapter
The Role of Stromal-Epithelial Interactions in the Regulation of Growth and Differentiation in Adult Epithelial Cells
There is general agreement that paracrine influences are of paramount importance during embryonic and fetal life. From studies on amphibians it has been shown that the differentiation of mesodermal tissue resu...
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Chapter
Mesenchymal-Epithelial Interactions in the Growth and Development of the Prostate
The ductal networks within the prostate originate from solid epithelial outgrowths (prostatic buds) that emerge from the endodermal urogenital sinus (UGS) immediately below the develo** bladder and grow into...
