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  1. No Access

    Protocol

    Efficient Generation of CRISPR/Cas9-Mediated Knockout Human Primary Keratinocytes by Electroporation

    Due to their full differentiation capacity in vitro, the culture of human primary keratinocytes (HPKs) represents a physiological model for answering basic biological and dermatological research questions, inc...

    Tugay Karakaya, Marta Slaufova, Michela Di Filippo, Paulina Hennig, Gabriele Fenini… (2024)

  2. No Access

    Protocol

    Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9

    The culture of epidermal human primary keratinocytes (HPKs) represents a well-established model in biological and dermatological research. In addition, HPKs are used in three-dimensional organotypic cultures (...

    Serena Grossi, Gabriele Fenini, Paulina Hennig, Michela Di Filippo… in Epidermal Cells (2020)

  3. Article

    Open Access

    Tumour hypoxia promotes melanoma growth and metastasis via High Mobility Group Box-1 and M2-like macrophages

    Hypoxia is a hallmark of cancer that is strongly associated with invasion, metastasis, resistance to therapy and poor clinical outcome. Tumour hypoxia affects immune responses and promotes the accumulation of ...

    Roman Huber, Barbara Meier, Atsushi Otsuka, Gabriele Fenini… in Scientific Reports (2016)

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