-
Article
An international optical microscopy event-“Focus on Microscopy 2010”
-
Article
Four-dimensional imaging of chromatin dynamics during the assembly of the interphase nucleus
Large-scale chromatin organization is likely to play an important role in epigenetic control of gene expression. This implies that after mitosis the correct chromatin organization must be re-established in the...
-
Chapter
Parametric Nonlinear Optical Techniques in Microscopy
A small revolution is taking place in microscopy. Nonlinear optical spectroscopic techniques — which in bulk applications have been around almost since the invention of the laser in the sixties — are rapidly b...
-
Chapter and Conference Paper
Ultrafast measurement of microscopic chemical environments using high intensity chirped pulses
High intensity chirped pulses are used to detect the pH of the environment of fluorophores, with possible application to confocal microscopy of living cells.
-
Chapter and Conference Paper
3D-microscopy using third-harmonic generation at interfaces in biological and non-biological specimens
3D imaging of the structure of transparent biological and non-biological samples is demonstrated using third harmonic generation at interfaces within the specimen.
-
Chapter
Real-Time Stereo (3D) Confocal Microscopy
Three-dimensional (3D) microscopy requires the collection of data over a certain volume in the specimen, followed by a suitable two-dimensional (2D) visualization procedure in which the desired image is produc...
-
Article
Confocal techniques for cell biology
-
Article
Turnover and transport of agglutinins in conjugatingChlamydomonas gametes
During gamete-gamete adhesion in the unicellular green algaChlamydomonas eugametos, the sexual adhesion molecules or agglutinins that are located on the flagella are subject to tip-oriented migration and rapid in...
-
Chapter
Size and Shape of The Confocal Spot: Control and Relation to 3D Imaging and Image Processing
A confocal microscope can be considered as a 3D sampling instrument for collecting data from spatial structures, especially biological ones. Optimal data collection in confocal microscopes requires the adaptat...
-
Article
Three-dimensional structure of living chloroplasts as visualized by confocal scanning laser microscopy
The newly developed confocal scanning laser microscope, together with image processing by computer, has been used to obtain three-dimensional information on the organization of grana in chloroplasts in living ...
-
Article
Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy
The relationship between cell shape and function has long been of interest1–9. However, although the behaviour of the cytoskeleton during the cell cycle has been studied extensively10–12 variations in the shape a...
-
Article
Bacterial anatomy in retrospect and prospect
Progress in bacterial anatomy over a period of about 15 years is reviewed. In particular, attention is paid to developments in which the Department of Electron Microscopy and Molecular Cytology was involved. P...
-
Article
Origin of Bright Spots in High Resolution Dark Field Electron Microscopy
SMALL bright spots (0.3–0.7 nm) have been observed in the dark field images of evaporated carbon films1 but their origin has not been explained. Here I suggest that these bright spots are present in the dark fiel...
