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Article
A pathogenic CtBP1 missense mutation causes altered cofactor binding and transcriptional activity
We previously reported a pathogenic de novo p.R342W mutation in the transcriptional corepressor CTBP1 in four independent patients with neurodevelopmental disabilities [1]. Here, we report the clinical phenotypes...
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Article
Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model
Human adenovirus (HAdV) vectors are intensely investigated for virotherapy of a wide variety of human cancers. Here, we have evaluated the effect of two apoptogenic HAdV5 vectors in an immunocompetent Syrian h...
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Article
Open AccessInteraction of ZEB and Histone Deacetylase with the PLDLS-binding cleft region of monomeric C-terminal Binding Protein 2
Proteins of the C-terminal binding protein (CtBP) family, CtBP1 and CtBP2 are closely related transcriptional regulators that are coded by two different gene loci in the vertebrate genomes. They perform redund...
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Article
BH3-only proteins in apoptosis and beyond: an overview
BH3-only BCL-2 family proteins are effectors of canonical mitochondrial apoptosis. They discharge their pro-apoptotic functions through BH1–3 pro-apoptotic proteins such as BAX and BAK, while their activity is...
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Article
BNIP3 subfamily BH3-only proteins: mitochondrial stress sensors in normal and pathological functions
The BNIP3 subfamily of BH3-only proteins consists of BNIP3 and BNIP3-like (BNIP3L) proteins. These proteins form stable homodimerization complexes that localize to the outer membrane of the mitochondria after ...
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Article
BIK, the founding member of the BH3-only family proteins: mechanisms of cell death and role in cancer and pathogenic processes
BIK is the founding member of the BH3-only family pro-apoptotic proteins. BIK is predominantly localized in the ER and induces apoptosis through the mitochondrial pathway by mobilizing calcium from the ER to t...
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Article
Inhibition of transcriptional activation and cell proliferation activities of adenovirus E1A by the unique N-terminal domain of CtBP2
The 243-residue E1A protein of adenovirus induces cellular proliferation, at least partly by regulating the transcription of cellular genes. This E1A function requires E1A N-terminal region and conserved regio...
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Article
BH3-only protein BIK induces caspase-independent cell death with autophagic features in Bcl-2 null cells
The BH3-only protein BIK normally induces apoptotic cell death. Here, we have investigated the role of BCL-2 in BIK-induced cell death using Bcl-2+/+ and Bcl-2−/− mouse embryo fibroblasts. Ectopic expression of B...
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Article
PLDLS-dependent interaction of E1A with CtBP: regulation of CtBP nuclear localization and transcriptional functions
C-terminal binding proteins (CtBPs) are cellular corepressors that are targeted by adenovirus E1A. A conserved motif of E1A (PLDLS) interacts with an N-terminal hydrophobic cleft of CtBPs. Many cellular cofact...
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Book
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Chapter
CtBP Family Proteins
CtBP family proteins are unique in animals and in plants. The invertebrates and plants contain a single CtBP family gene while vertebrates have two genes. Genetic studies in Drosophila and in mice indicate that C...
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Article
Bak functionally complements for loss of Bax during p14ARF-induced mitochondrial apoptosis in human cancer cells
In contrast to the initial notion that the biological activity of p14ARF strictly depends on a functional mdm-2/p53 signaling axis, we recently demonstrated that p14ARF mediates apoptosis in a p53/Bax-independent...
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Article
Mitochondrial localization of p53 during adenovirus infection and regulation of its activity by E1B-19K
Recent results have revealed that the p53 tumor suppressor protein possesses a direct transcription-independent apoptotic activity. During apoptosis induced by genotoxic stress, a small fraction of p53 is targ...
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Chapter
Modulation of Oncogenic Transformation by the Human Adenovirus E1A C-Terminal Region
The E1A oncogene of human adenoviruses cooperates with other viral and cellular oncogenes in oncogenic transformation of primary and established cells. The N-terminal half of E1A proteins that form specific prote...
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Protocol
Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein E1B-19K
Human adenoviruses have been excellent models for gene expression in mammalian cells. They have also been very important tools in dissecting complex cellular processes such as control of cell proliferation, on...
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Article
Functional identification of the apoptosis effector BH3 domain in cellular protein BNIP1
BCL-2 family proteins play a central role in apoptosis regulation in mammals and in C. elegans. Mammalian cellular and viral anti-apoptosis proteins such as BCL-2 and E1B-19K interact with several cellular protei...
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Protocol
Determination of the Transforming Activities of Adenovirus Oncogenes
Oncogenesis is a multistep process that ultimately leads to the demise of the organism. These steps can be mimicked in vitro by treatment of cultured animal cells with various oncogenic agents such as chemical...
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Protocol
Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members
Programmed cell death, or apoptosis, is an important event in the normal development and homeostasis of multicellular organisms (1). Apoptosis is regulated by a number of genes that promote or suppress cell death...
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Article
Regulation of apoptosis by a Caenorhabditis elegans BNIP3 homolog
We have identified a C. elegans protein, ceBNIP3, homologous to the human BCL-2/EIB-19K interacting BCL-2 family pro-apoptotic protein BNIP3. In transiently transfected mammalian cells, ceBNIP3 complexes with CED...
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Article
Functional dissection of Bfl-1, a Bcl-2 homolog: anti-apoptosis, oncogene-cooperation and cell proliferation activities
The human Bfl-1 gene codes for a 175-amino acid BCL-2 family protein that has an anti-apoptosis activity and is overexpressed in certain human epithelial and hematopoietic malignancies. Bfl-1 efficiently suppress...
