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Article
Regulation of cytoplasmic pH of cultured bovine corneal endothelial cells in the absence and presence of bicarbonate
Intracellular pH (pH i ) in confluent monolayers of cultured bovine corneal endothelial cells was determined using the pH-dependent absorbance of intracellularly trapped 5(and 6)...
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Article
Evidence for Na/H exchange and Cl/HCO3 exchange in A10 vascular smooth muscle cells
In the present study we used the pH sensitive absorbance of 5(and6)-carboxy-4′,5′-dimethylfluorescein to investigate intracellular pH (pHi) regulation in A10 vascular smooth muscle cells: (1) The steady state pHi
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Article
Na+-dependent HCO 3 − transport and Na+/H+ exchange regulate pH i in human ciliary muscle cells
We investigated intracellular pH (pH i ) regulation in cultured human ciliary muscle cells by means of the pH-sensitive absorbance of 5(and 6)-carboxy-4′,5′-dimethylfluorescein (...
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Article
Characterization of acetylcholine- and endothelin-induced calcium entry in cultured human ciliary muscle cells
We characterized the effects of acetylcholine and endothelin on cultured human ciliary muscle cells, using the calcium-sensitive dye fura-2 to measure intracellular calcium and intracellular microelectrodes to...
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Chapter
Future Aspects of Bioprocess Monitoring
Nature has the impressive ability to efficiently and precisely control biological processes by applying highly evolved principles and using minimal space and relatively simple building blocks. The challenge...
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Article
Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression
Characterizing and understanding the functional heterogeneity in a given population on the cellular and molecular level is a great challenge in microbiology. Each microorganism contributes differently to the o...
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Chapter and Conference Paper
A Preliminary Study on Spider Silk as Biomaterial for Peripheral Nerve Regeneration
Biomaterials for Tissue Engineering applications have to be biocompatible and biodegradable, they should enhance cell adhesion and enable sufficient nutrient transport. Moreover, in regard to nerve repair they ha...
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Chapter and Conference Paper
A Study on Bioscaffolds of Polysialic Acid and β-Glucan for Cell Culture and Tissue Engineering Applications
Biopolymers play an important role for cell culture and tissue engineering. The inherent biodegradability and biocompatibility of the biopolymers are important regulation factors for cell and tissue remodellin...
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Article
Open AccessLaser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers
Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context, in situ conjugation of nanoparticles with biomolecules via laser ablation in an aque...
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Article
Anwendungen von Aptameren in der Mikroarray-Technologie
Die Anwendung von Aptameren in der Mikroarray-Technik ist eine neue, vielversprechende Methode zur Detektion von Proteinen. Aptamer-Mikroarrays können zudem als Screening-Tools für die Untersuchung der Aptamer...
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Article
Growth and Differentiation Properties of Mesenchymal Stromal Cell Populations Derived from Whole Human Umbilical Cord
Up to 2.8 × 107 fibroblast-like cells displaying an abundant presence of mesenchymal stem cell (MSC) markers CD73, CD90, CD105 and a low level of HLA-I expression can be isolated from one whole human umbilical co...
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Chapter and Conference Paper
A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells
Embryonic Stem (ES) cells are self- renewing and pluripotent cells that can differentiate into a variety of cell lineages. Implementation in tissue engineering or as model systems for drug discovery makes ES c...
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Chapter and Conference Paper
A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture
The screening of high-producing cells is a time consuming procedure. Starting from a single cell after a single cell dilution the procedure takes weeks to complete. The affinity matrix method is a technique fo...
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Chapter
Transcriptome Analysis
Transcriptome analysis technologies are important systems-biology methods for the investigation and optimization of mammalian cell cultures concerning with regard to growth rates and productivity. For the prod...
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Article
Open AccessTranscriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effec...
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Article
Open AccessDifferent gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: results from a randomized controlled trial
Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in hu...
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Article
Open AccessRegulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are large...
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Chapter
Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis
Individuals within natural populations are highly diverse. They can vary regarding their physiological states and thus contribute differently to the performance of the whole community [1]. Hence, the character...
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Chapter
Aptamer-Modified Nanoparticles as Biosensors
Aptamers are short oligonucleotides that are capable of selectively binding to their corresponding target. Therefore, they can be thought of as a nucleic acid-based alternative to antibodies and can substitute...
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Chapter
Molecular Survival Strategies of Organisms: HSP and Small Molecules for Diagnostics and Drug Development
Nature has developed evolutionary strategies to synthesize the vast number of what are called secondary metabolites or in short natural products. These include molecules that are able to inhibit heat shock pro...
