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Article
PARP inhibition leads to synthetic lethality with key splicing-factor mutations in myelodysplastic syndromes
Splicing factors are frequently mutated in patients with myelodysplastic syndromes and acute myeloid leukaemia. Recent studies have revealed convergent molecular defects caused by splicing factor mutations, am...
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Article
Certified by social networks: the role of social certifications in medical crowdfunding
Nowadays, medical crowdfunding platforms are faced with two challenges: the trustworthiness issue caused by inauthentic information provided by fundraisers and the trackability issue caused by insufficient inf...
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Article
Open AccessIron-sulfur clusters are involved in post-translational arginylation
Eukaryotic arginylation is an essential post-translational modification that modulates protein stability and regulates protein half-life. Arginylation is catalyzed by a family of enzymes known as the arginyl-t...
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Protocol
Assaying Arginylation Activity in Cell Lysates Using a Fluorescent Reporter
Here, we describe an antibody-based method to evaluate the enzymatic activity of arginyltransferase1 (Ate1). The assay is based on the arginylation of a reporter protein, which contains the N-terminal peptide ...
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Article
A spatiotemporal analysis of extreme agrometeorological events during selected growth stages of maize (Zea mays L.) from 1960 to 2017 in Northeast China
Against the background of global warming, extreme agrometeorological events have become more intense and frequent during the growing season of maize (Zea mays L.). In this study, we focused on the five types of e...
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Article
Spatial Distribution and Temporal Trend Characteristics of Agro-Climatic Resources and Extreme Climate Events during the Soybean Growing Season in Northeast China from 1981 to 2017
Soybean is an important oil crop. Agro-climatic resources and extreme climate events during the growing season directly affect the crop growth and grain yield. In this study, we used historical climate data an...
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Article
Open AccessInsertion/deletion-activated frame-shift fluorescence protein is a sensitive reporter for genomic DNA editing
Reporter methods to quantitatively measure the efficiency and specificity of genome editing tools are important for the development of novel editing techniques and successful applications of available ones. Ho...
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Article
Reduced Arginyltransferase 1 is a driver and a potential prognostic indicator of prostate cancer metastasis
Most prostate cancer cases remain indolent for long periods of time, but metastatic progression quickly worsens the prognosis and leads to mortality. However, little is known about what promotes the metastasis...
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Article
Open AccessProteomic and microRNA Transcriptome Analysis revealed the microRNA-SmyD1 network regulation in Skeletal Muscle Fibers performance of Chinese perch
Fish myotomes are comprised of anatomically segregated fast and slow muscle fibers that possess different metabolic and contractile properties. Although the expression profile properties in fast and slow muscl...
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Article
Open AccessPosttranslational arginylation enzyme Ate1 affects DNA mutagenesis by regulating stress response
Arginyltransferase 1 (Ate1) mediates protein arginylation, a poorly understood protein posttranslational modification (PTM) in eukaryotic cells. Previous evidence suggest a potential involvement of arginylatio...
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Article
Arginylation regulates purine nucleotide biosynthesis by enhancing the activity of phosphoribosyl pyrophosphate synthase
Protein arginylation is an emerging post-translational modification that targets a number of metabolic enzymes; however, the mechanisms and downstream effects of this modification are unknown. Here we show tha...
