11 Result(s)
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Esmerilda G. Delicado
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Article
Open AccessDUSP1/MKP-1 represents another piece in the P2X7R intracellular signaling puzzle in cerebellar cells: our last journey with Mª Teresa along the purinergic pathways of Eden
The P2X7 receptor (P2X7R) stands out within the purinergic family as it has exclusive pharmacological and regulatory features, and it fulfills distinct roles depending on the type of stimulation and cellular e...
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Protocol
Intracellular Calcium Recording After Purinoceptor Activation Using a Video-Microscopy Equipment
Calcium is one of the most important intracellular messengers, triggering a wide range of cellular responses. Changes in intracellular free calcium concentration can be measured using calcium sensitive fluores...
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Protocol
Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro
A comprehensive understanding of the mechanisms controlling the behavior of cell populations with regenerative potential is the first step to design effective therapeutic strategies for many diseases. However,...
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Chapter
An Update on P2Y13 Receptor Signalling and Function
The distribution of nucleotide P2Y receptors across different tissues suggests that they fulfil key roles in a number of physiological and pathological conditions. P2Y13 is one of the latest P2Y receptors identif...
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Article
Open AccessP2X7, NMDA and BDNF receptors converge on GSK3 phosphorylation and cooperate to promote survival in cerebellar granule neurons
Glycogen synthase kinase-3 (GSK3) is a key player in the regulation of neuronal survival. Herein, we report evidence of an interaction between P2X7 receptors with NMDA and BDNF receptors at the level of GSK3 s...
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Article
Open AccessP2X7 Nucleotide Receptor is Coupled to GSK-3 Inhibition and Neuroprotection in Cerebellar Granule Neurons
In this study we report the coupling of nucleotide receptors to GSK-3 signalling, a relevant survival pathway in cerebellar granule neurons. P2X7 agonist BzATP induced a 3–4-fold increase in GSK-3 phosphorylation...
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Article
Dinucleoside polyphosphates and their interaction with other nucleotide signaling pathways
Dinucleoside polyphosphates or ApnA are a family of dinucleotides formed by two adenosines joined by a variable number of phosphates. Ap4A, Ap5A, and Ap6A are stored together with other neurotransmitters into sec...
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Chapter
Allosteric Modulation of Nucleoside Transport by Adenosine and ATP
Adenosine levels in extracellular fluids depend on a large set of physiologic or pathologic events, and there is broad evidence for a cardiovascular and neuroprotective role of this nucleoside in hypoxic or is...
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Chapter
Extracellular Signals and Transduction Mechanisms Controlling the Adenosine Transport in Neural Cells
The adenosine transport in bovine chromaffin cells is highly regulated by different extracellular signals, both at short and long-term. The short-term regulation by extracellular signals such as secretagogues ...
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Chapter
Control of Nucleoside Transport in Neural Cells Effect of Protein Kinase C Activation
Although nucleoside transport has been extensively characterized in synaptosomal preparations, they are not suitable for regulatory studies because the cellular integrity is necessary1,2. Adenosine transport is a...
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Article
Modulation of the kinetic characteristics of the sarcoplasmic reticulum ATPase by membrane fluidity
(Ca2+ + Mg2+)-ATPase from sarcoplasmic reticulum has been reconstituted with dipalmitoylphosphatidylcholine, and the activating effect of ATP and Ca2+ on this enzyme has been studied at different temperatures. It...
