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7 Result(s)
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Book
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Protocol
A Brief Introduction to Avian Influenza Virus
Avian influenza virus (AIV) causes a disease of high economic importance for poultry production worldwide. The earliest recorded cases of probable high-pathogenicity AIV in poultry were reported in Italy in th...
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Protocol
Influenza Subtype Identification with Molecular Methods
Gene sequencing and RT-PCR based methods are the molecular alternative to serology for the identification of influenza virus hemagglutinin and neuraminidase antigenic subtypes. Compared to serology both RT-PCR...
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Protocol
Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR
Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s for routine surveillance, outbreak assessment and for research. Some of the advantages of rRT-PCR are: h...
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Protocol
Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs
Avian influenza virus and some mammalian influenza A viruses are usually isolated, propagated, and titrated in embryonated chicken eggs (ECE). Most any sample type can be accommodated for culture with appropri...
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Protocol
Avian Influenza Virus RNA Extraction
The efficient extraction and purification of viral RNA is critical for down-stream molecular applications whether it is the sensitive and specific detection of virus in clinical samples, virus gene cloning and...
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Protocol
Detection of Influenza A Antibodies in Avian Serum Samples by ELISA
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to...
