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Article
Quorum-sensing based small RNA regulation for dynamic and tuneable gene expression
Develo** a dynamic regulation strategy is an essential step in establishing an automatic control system for manipulating metabolic fluxes and cellular behaviors. To broaden the extent of the application, a s...
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Article
Molecular Dynamics Simulation Reveals Unique Interplays Between a Tarantula Toxin and Lipid Membranes
Tarantula toxins compose an important class of spider toxins that target ion channels, and some are known to interact with lipid membranes. In this study, we focus on a tarantula toxin, **gzhaotoxin-III (JZTx...
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Article
Open AccessOEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments
Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on O verlap E xtension P CR and R ecom...
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Article
Open AccessScreening for Voltage-Gated Sodium Channel Interacting Peptides
The voltage-gated sodium channel (VGSC) interacting peptide is of special interest for both basic research and pharmaceutical purposes. In this study, we established a yeast-two-hybrid based strategy to detect...