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Open AccessA CAF40-binding motif facilitates recruitment of the CCR4-NOT complex to mRNAs targeted by Drosophila Roquin
Human (Hs) Roquin1 and Roquin2 are RNA-binding proteins that promote mRNA target degradation through the recruitment of the CCR4-NOT deadenylase complex and are implicated in the prevention of autoimmunity. Roqui...
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Structure of the Dcp2–Dcp1 mRNA-decap** complex in the activated conformation
Crystal structures, along with biochemistry data, capture the active form of the Dcp2 decap** enzyme in complex with Dcp1 and a peptide from Edc1, thus revealing the mechanism of activation by Dcp1 and Edc1 ...
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Towards a molecular understanding of microRNA-mediated gene silencing
MicroRNAs (miRNAs) silence gene expression by repressing translation and accelerating target mRNA degradation.
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Erratum: An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay
Nat. Struct. Mol. Biol. 21, 599–608 (2014); published online 1 June 2014; corrected after print 22 August 2014. In the version of this article initially published, a rotation sign was mistakenly introduced bet...
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Open Access4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation
eIF4E-binding proteins (4E-BPs) are a widespread class of translational regulators that share a canonical (C) eIF4E-binding motif (4E-BM) with eIF4G. Consequently, 4E-BPs compete with eIF4G for binding to the ...
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An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay
Structural analyses reveal the asymmetric assembly of Neurospora crassa PAN2–PAN3 complex and, along with functional work on the proteins from different species, indicate an essential role for PAN3 in coordinatin...
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Structure and assembly of the NOT module of the human CCR4–NOT complex
The CCR4–NOT deadenylase complex has a crucial role in post-transcriptional mRNA regulation, catalyzing the removal of mRNA poly(A) tails and consequently repressing translation and promoting mRNA degradation....
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Making sense of nonsense
Eukaryotic mRNAs containing nonsense codons are degraded by nonsense-mediated mRNA decay (NMD). In humans, NMD was proposed to act exclusively on newly synthesized mRNA during a 'pioneer round' of translation,...
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A role for eIF4AII in microRNA–mediated mRNA silencing
A recent study on the mechanism of microRNA–mediated gene silencing suggests that microRNA–induced silencing complexes inhibit ribosome scanning by recruiting the DEAD-box RNA helicase eIF4AII through an inter...
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The Role of GW182 Proteins in miRNA-Mediated Gene Silencing
GW182 family proteins are essential for microRNA-mediated gene silencing in animal cells. They are recruited to miRNA targets through direct interactions with Argonaute proteins and promote target silencing. T...
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A direct interaction between DCP1 and XRN1 couples mRNA decap** to 5′ exonucleolytic degradation
The removal of the mRNA 5′ cap structure by the decap** enzyme DCP2 leads to rapid 5′→3′ mRNA degradation by XRN1, suggesting that the two processes are coordinated. Biochemical and structural analyses now r...
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Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP
The TAP-p15 heterodimer is involved in nuclear export of messenger RNAs. The crystal structure of TAP (RRM + LRR domains) bound to part of the export element found in simian type D retroviral RNAs, together wi...
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Gene silencing by microRNAs: contributions of translational repression and mRNA decay
MicroRNAs (miRNAs) repress the expression of mRNA targets by promoting translational repression and mRNA degradation.
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Role of GW182 proteins and PABPC1 in the miRNA pathway: a sense of déjà vu
GW182 proteins are key components of microRNA silencing complexes in animals, although their precise molecular function has been poorly understood. Recent findings indicate that they promote gene silencing by ...
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Freedom versus constraint in protein function
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GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay
MicroRNAs (miRNAs) silence gene expression by binding 3′ untranslated regions of target mRNAs. Recent studies suggested silencing is achieved through either recruitment of eIF6, which prevents ribosome assembl...
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P bodies: at the crossroads of post-transcriptional pathways
P bodies are discrete cytoplasmic domains in which proteins that are involved in mRNA degradation, translational repression, mRNA surveillance and RNA-mediated...
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Generation and annotation of the DNA sequences of human chromosomes 2 and 4
Human chromosome 2 is unique to the human lineage in being the product of a head-to-head fusion of two intermediate-sized ancestral chromosomes. Chromosome 4 has received attention primarily related to the sea...
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The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin α
Addition of N-acetylglucosamine (GlcNAc) is a ubiquitous form of intracellular glycosylation catalyzed by the conserved O-linked GlcNAc transferase (OGT). OGT contains an N-terminal domain of tetratricopeptide (T...
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Letter to the Editor: NMR Assignment of the Drosophila Argonaute2 PAZ Domain
