-
Protocol
Nontargeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS
d-amino acid-containing peptides (DAACPs) in animals are a class of bioactive molecules formed via the posttranslational modification of peptides consisting of all-l-amino acid residues. Amino acid residue isomer...
-
Protocol
Bioinformatics for Prohormone and Neuropeptide Discovery
Neuropeptides and peptide hormones are signaling molecules produced via complex posttranslational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are ass...
-
Article
Open AccessBiodistribution and racemization of gut-absorbed l/d-alanine in germ-free mice
Microbiome-derived metabolites are important for the microbiome-gut-brain axis and the discovery of new disease treatments. d-Alanine (d-Ala) is found in many animals as a potential co-agonist of the N-methyl-d-a...
-
Article
Peptidomics
Peptides are biopolymers, typically consisting of 2–50 amino acids. They are biologically produced by the cellular ribosomal machinery or by non-ribosomal enzymes and, sometimes, other dedicated ligases. Pepti...
-
Article
Open AccessTranscriptomic plasticity of the hypothalamic osmoregulatory control centre of the Arabian dromedary camel
Water conservation is vital for life in the desert. The dromedary camel (Camelus dromedarius) produces low volumes of highly concentrated urine, more so when water is scarce, to conserve body water. Two hormones,...
-
Protocol
High-Throughput Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells
Characterizing the chemicals within individual cells is an important analytical capability, with mass spectrometry (MS) being one of the most chemically information-rich techniques available. There are two maj...
-
Article
Open AccessImage-guided MALDI mass spectrometry for high-throughput single-organelle characterization
Peptidergic dense-core vesicles are involved in packaging and releasing neuropeptides and peptide hormones—critical processes underlying brain, endocrine and exocrine function. Yet, the heterogeneity within th...
-
Chapter and Conference Paper
Engineering as a Coordinating Method for the Development of the Organization and Society
The article analyzes the development of concept “engineering” and substantiates its role as an innovative coordinating method for the further development of automation systems to control the functioning proces...
-
Article
Open AccessGenetic diversity of SAD and FAD genes responsible for the fatty acid composition in flax cultivars and lines
Flax (Linum usitatissimum L.) is grown for fiber and seed in many countries. Flax cultivars differ in the oil composition and, depending on the ratio of fatty acids, are used in pharmaceutical, food, or paint ind...
-
Chapter and Conference Paper
Transformation PLM-Systems into the Cyber-Physical Systems for the Information Provision for Enterprise Management
The article proposes a concept of transforming the existing Product Lifecycle Management System (PLM-System) into a Cyber-Physical System for the production and management of an enterprise (in an extended mea...
-
Protocol
Neuropeptidomics of the Mammalian Brain
A suite of bioactive peptides orchestrates a variety of cellular interactions in the mammalian brain. The bioanalytical strategy known as neuropeptidomics evolved from the quest to globally characterize these ...
-
Article
Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry
Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made in peptide identification across the Metazoa, for some ...
-
Chapter and Conference Paper
Stress Co** Strategies in University Students
Psychological stress accompanies many typical activities of the students including examination, assignments and projects deadlines, prospective job’s practice applications etc. Individual stress co** strateg...
-
Protocol
Bioinformatics for Prohormone and Neuropeptide Discovery
Neuropeptides and peptide hormones are signaling molecules produced via complex post-translational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are as...
-
Article
Open AccessEvolution of mitochondrial genomes in Baikalian amphipods
Amphipods (Crustacea) of Lake Baikal are a very numerous and diverse group of invertebrates generally believed to have originated by adaptive radiation. The evolutionary history and phylogenetic relationships ...
-
Article
Open AccessIdentification of prohormones and pituitary neuropeptides in the African cichlid, Astatotilapia burtoni
Cichlid fishes have evolved remarkably diverse reproductive, social, and feeding behaviors. Cell-to-cell signaling molecules, notably neuropeptides and peptide hormones, are known to regulate these behaviors a...
-
Article
Peptidomics and Secretomics of the Mammalian Peripheral Sensory-Motor System
The dorsal root ganglion (DRG) and its anatomically and functionally associated spinal nerve and ventral and dorsal roots are important components of the peripheral sensory-motor system in mammals. The cells w...
-
Article
Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain
Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and eng...
-
Article
Profiling metabolites and peptides in single cells
The intracellular levels and spatial localizations of metabolites and peptides reflect the state of a cell and its relationship to its surrounding environment. Moreover, the amounts and dynamics of metabolites...
-
Protocol
Neuropeptidomics of the Mammalian Brain
A suite of bioactive peptides orchestrates a variety of cellular interactions in the mammalian brain. A new bioanalytical strategy, neuropeptidomics, has evolved from the quest to characterize these important ...
