13 Result(s)
within
Elena Conti
Biochemistry, general
Membrane Biology
Protein Structure
-
Article
The hitchhiker's guide to the nucleus
The hormone-related protein PTHrP travels from the cytosol to the nucleus by binding to the transport factor importin β. Remarkably, the site of recognition of PTHrP is the N-terminal half of importin β, which...
-
Article
A novel mode of RBD-protein recognition in the Y14–Mago complex
Y14 and Mago are conserved eukaryotic proteins that associate with spliced mRNAs in the nucleus and remain associated at exon junctions during and after nuclear export. In the cytoplasm, Y14 is involved in mRN...
-
Article
The superhelical TPR-repeat domain of O-linked GlcNAc transferase exhibits structural similarities to importin α
Addition of N-acetylglucosamine (GlcNAc) is a ubiquitous form of intracellular glycosylation catalyzed by the conserved O-linked GlcNAc transferase (OGT). OGT contains an N-terminal domain of tetratricopeptide (T...
-
Article
The archaeal exosome core is a hexameric ring structure with three catalytic subunits
The exosome is a 3′ → 5′ exoribonuclease complex involved in RNA processing. We report the crystal structure of the RNase PH core complex of the Sulfolobus solfataricus exosome determined at a resolution of 2.8 Å...
-
Article
Dicer measures up
Recent structures of Dicer and the related bacterial enzyme, RNase III, demonstrate how Dicer acts as a molecular ruler, measuring out a precise length of double stranded RNA before cleavage.
-
Article
Structural insights into the Notch-modifying glycosyltransferase Fringe
Fringe proteins are β1,3-N-acetylglucosaminyltransferases that modify Notch receptors, altering their ligand-binding specificity to regulate Notch signaling in development. We present the crystal structure of mou...
-
Article
A single subunit, Dis3, is essentially responsible for yeast exosome core activity
The conserved core of the exosome, the major eukaryotic 3′ → 5′ exonuclease, contains nine subunits that form a ring similar to the phosphorolytic bacterial PNPase and archaeal exosome, as well as Dis3. Dis3 i...
-
Article
The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner
The DEAD-box protein DBP5 is involved in yeast mRNA export, though the mechanism by which it helps to remodel and release transcripts on the cytoplasmic face of the nuclear pore complex has been unclear. The s...
-
Article
The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex
The ASAP complex is emerging as an assembly of proteins at the interface between transcription, pre-mRNA splicing and mRNA quality control. Structural analysis of the ASAP core complex reveals macromolecular i...
-
Article
The cryo-EM structure of the UPF–EJC complex shows UPF1 poised toward the RNA 3′ end
The nonsense-mediated mRNA decay (NMD) pathway is triggered upon assembly of the UPF surveillance complex near an exon junction complex (EJC). Cryo-EM studies have revealed the geometry of this transient assem...
-
Article
Structure and RNA-binding properties of the Not1–Not2–Not5 module of the yeast Ccr4–Not complex
The Ccr4–Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. Structural, biochemical and functional analyses of the Not module, comp...
-
Article
The structure of the Pan2–Pan3 core complex reveals cross-talk between deadenylase and pseudokinase
Conti and colleagues present the crystal structure of the yeast deadenylase Pan2–Pan3 core complex, revealing a 1:2 stoichiometry and indicating how deadenylase and pseudokinase domains work together to promot...
-
Article
InsP6 binding to PIKK kinases revealed by the cryo-EM structure of an SMG1–SMG8–SMG9 complex
We report the 3.45-Å resolution cryo-EM structure of human SMG1–SMG8–SMG9, a phosphatidylinositol-3-kinase (PI(3)K)-related protein kinase (PIKK) complex central to messenger RNA surveillance. Structural and M...
