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Article
Comparison of Alleles at Gli-2 Loci of Common Wheat by Means of Two-Dimensional Electrophoresis of Gliadin
Electrophoretic mobility (EM) and molecular weight (MW) of some allelic variants of α- and β-gliadins contrlled by Gli-2 loci were compared by means of two-dimensional (APAGE × SDS) electrophoresis. Comparison of...
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Article
Comparison of alleles at the Gli-1 loci of common wheat by means of two-dimensional electrophoresis of gliadin and RFLP analysis
Allelic variants of the Gli-1 locus is known to control groups (blocks) of gliadin polypeptides (gliadins). Some allelic variants of blocks that differ in the electrophoretic (acid gel) mobility (EM) of only one ...
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Article
Assessment of storage protein variation in relation to some morphological characters in a sample of Spanish landraces of common wheat (Triticum aestivum L. ssp. aestivum)
Fifty nine Spanish landraces of common wheat belonging to differentagrotypes were evaluated with four morphological spike characters and 11 seedprotein loci in order to study the level of agreement between the...
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Article
Genetic diversity of French common wheat germplasm based on gliadin alleles
Analysis of gliadin electrophoretic (APAGE) patterns made it possible to identify 79 alleles at six Gli-1 and Gli-2 loci (from 9 to 18 per locus) and 173 gliadin genotypes in the 187 French common wheat cultivar...
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Article
Recombination map** of some chromosome 1A-, 1B-, 1D- and 6B-controlled gliadins and low-molecular-weight glutenin subunits in common wheat
Inheritance of low-molecular-weight glutenin subunits (LMW GS) and gliadins was studied in the segregating progeny from several crosses between common wheat genotypes. The occurrence of a few recombinants in ...
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Article
Production and genetic characterization of near-isogenic lines in the bread-wheat cultivar Alpe
Two biotypes of the bread-wheat cultivar Alpe were shown to possess contrasting alleles at each of the glutenin (Glu-B1, Glu-D1, Glu-B3 and Glu-D3) and gliadin (Gli-B1 and Gli-D1) loci on chromosomes 1B and 1D. F...
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Article
Genetic variation at storage protein-coding loci of common wheat (cv ‘Chinese Spring’) induced by nitrosoethylurea and by the cultivation of immature embryos in vitro
Electrophoretic patterns of seed storage proteins, the high-molecular-weight glutenins and gliadins, were studied in 468 plants of the common wheat cultivar ‘Chinese Spring’ regenerated from callus culture of ...
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Article
RFLP patterns of gliadin alleles in Triticum aestivum L.: implications for analysis of the organization and evolution of complex loci
A correspondence between RFLP patterns and gliadin alleles at the Gli-1 and Gli-2 loci was established in a set of 70 common wheat (T.aestivum L.) cultivars using γ-gliadin (K32) and α-gliadin (pTU1) specific pro...
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Article
Recombination map** of Gli-5, a new gliadin-coding locus on chromosomes 1A and 1B in common wheat
Inheritance studies of gliadin loci on chromosomes 1A and 1B were carried out in the progeny from crosses between cv “Salmone” and six other common wheat varieties. The map distance between the Rg-1 locus for glu...
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Article
Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa
The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by si...
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Article
Polymorphism and inheritance of gliadin polypeptides in T. monococcum L.
More than 80 different gliadin electrophoretic patterns (spectra) have been found in 109 accessions of the diploid wheat Triticum monococcum. Each pattern consists of 15–20 gliadin bands. Some patterns are clearl...
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Article
Chromosomal control of wheat gliadin protein epitopes: analysis with specific monoclonal antibodies
The genetic relationships between small clusters of monomeric alcohol-soluble wheat (Triticum aestivum L.) grain storage proteins (gliadins) were studied using a panel of monoclonal antibodies and immunoblotting,...
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Article
Gliadin allele composition of Yugoslav winter wheat cultivars
The complete gliadin allele composition of 57 Yugoslav common winter wheat cultivars was studied. Large differences were found in gliadin genotypes among cultivars bred at different Yugoslav breeding centres a...
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Article
The high-molecular-weight glutenin subunit composition of Soviet wheat varieties
One hundred and twenty-eight wheat varieties bred in the Soviet Union were screened for the composition of high-molecular-weight (HMW) subunits of glutenin. In general, variability was low compared to that see...
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Chapter
Genetics of Gliadin Proteins and the Problems of Interpreting Results Obtained with Somaclonal Variation in Wheat
Seed storage alcohol-soluble protein of wheat — gliadin is apparently one of the most heterogeneous proteins in the plant kingdom. Each genotype as a rule has a unique set of gliadin polypeptides which can be ...
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Article
Analysis of phylogenetic relations of durum, carthlicum and common wheats by means of comparison of alleles of gliadin-coding loci
Polymorphism and inheritance of wheat storage protein, gliadin, of durum (macaroni) and carthlicum wheats have been studied. Analysis of gliadin in 78 cultivars and in F2 seeds of intercultivar crosses of durum w...
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Article
Polymorphism and inheritance of gliadin components controlled by chromosome 6A of spring durum wheat
The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate...
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Article
Polymorphism and inheritance of gliadin components controlled by chromosome 6A of spring durum wheat
The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate...
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Article
Problems of interpreting results obtained in studies of somaclonal variation in gliadin proteins in wheat
It is quite likely that somacloning increases the frequency of mutations: this has, in fact, been claimed for gliadin proteins. However, in such instances it is important to distinguish between true mutations ...
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Article
Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes
Analysis of F2 grains from two different crosses has revealed a complex organization of the family of gliadin-coding genes located on chromosomes of the first homoeological group in hexaploid wheat. Chromosome 1A...
