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Article
Cytogenetic Analysis of the Results of Genome Editing on the Cell Model of Parkinson’s Disease
We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson’s disease. Genome editing was performed on induced pluripotent stem cells de...
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Article
Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine and N-docosahexaenoyl dopamine examined in neuronal precursors derived from human pluripotent stem cells
Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine (NADA) and N-docosahexaenoyl dopamine (DHDA) were examined in neuronal precursor cells differentiated from human induced pluripotent stem ...
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Article
Effect of Nerve Growth Factor on Neural Differentiation of Mouse Embryonic Stem Cells
The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of ...
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Article
Investigation of the effects of GABA receptor agonists in the differentiation of human induced pluripotent stem cells into dopaminergic neurons
The influence of GABA receptor agonists on the terminal differentiation in vitro of dopaminergic (DA) neurons derived from IPS cells was investigated. GABA-A agonist muscimol induced transient elevation of int...
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Article
Fibroblast-like cells as an effective feeder for the cultivation and derivation of new lines of human induced pluripotent stem cells
Induced pluripotent stem cells (iPSCs) can be a highly informative model of hereditary and sporadic human diseases. In the future, such cells can be used in substitution and regenerative therapy of a wide rang...
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Article
Investigation of the effect of α-melanocyte-stimulating hormone on proliferation and early stages of differentiation of human induced pluripotent stem cells
We have studied the influence of α-melanocyte-stimulating hormone (α-MSH) on proliferation and early stages of differentiation of human induced pluripotent stem cells (iPSc). We have demonstrated that α-MSH re...
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Article
The effect of α-melanocyte-stimulating hormone on early stages of differentiation of mouse embryonic stem cells
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Article
Effect of the expression of the human pub gene on the proliferation and differentiation of rat pheochromocytoma PC-12 cells
We studied the effect of overexpression of the human pub gene on the proliferation and neuronal differentiation of PC-12 cells treated with nerve growth factor. We obtained a stable cell line overexpressing the h...
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Article
Different Effects of Enhanced and Reduced Expression of pub Gene on the Formation of Embryoid Bodies by Cultured Embryonic Mouse Stem Cell
The effects of pub gene on proliferation and initial stages of differentiation of embryonic mouse stem cells were studied in vitro. To this end we used enhanced expression of human pub gene (hpub) and suppression...
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Article
BFP Protein Expression in Transfected Embryonic Stem Cells
Cultured mouse embryonic stem cells can be transfected with a reporter gene encoding blue fluorescent protein BFP and regulated by drosophila heat shock protein 70 promoter. This gene is activated after heatin...
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Article
Study of the activity of calcium channels that are regulated by intracellular calcium stores in mouse embryonic stem cells
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Article
Expression Analysis of Several Lymphoma-specific Genes in Human Fibroblasts Immortalized with SV40
Subtraction hybridization was earlier used to obtain cDNA clones corresponding to human genes upregulated in HIV-associated centroblast lymphoma (CL) as compared with HIV-associated immunoblast lymphoma (IL). ...
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Article
Differentiation of Pluripotent Embryonic Stem Cells in the Peritoneal Cavity of Irradiated Mice
We studied the behavior and differentiation of pluripotent embryonic stem cells of R1 mice in vivo. Undifferentiated embryonic stem cells and differentiating embryoid bodies implanted in the abdominal cavity of i...
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Article
Influence of Regulatory Genes of Type 1 Human Immunodeficiency Virus on Proliferation and Differentiation of Murine Embryonic Stem Cells
Spontaneous formation of embryoid bodies and subsequent differentiation of some cells into cardiomyocytes were demonstrated on murine embryonic stem cells of R1 line. The lines of embryonic stem cells were obt...
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Article
Expression of Regulatory GenesOct-4, Pax-6, Prox-1, and Ptx-2 at the Initial Stages of Differentiation of Embryonic Stem Cells in vitro
The expression of regulatory genes of the POU, Pax, Prox, and Ptx gene families was studied at the initial stages of differentiation of murine embryonic stem cells of R1 line. mRNAs were isolated from undifferent...
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Article
Characteristics of a Pluripotent Population at Early Stages of Embryonic Stem Cell Differentiation in Culture
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Article
Embryonic Stem Cells: Spontaneous and Directed Differentiation
The specific structural features of embryonic stem cells and embryoid bodies and mechanisms of their differentiation in different cell types are considered. The mouse embryonic stem cells (line R1) formed mult...
