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Chapter
Metabolic Engineering
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Article
Enzymatic production of cyclodextrins
Cyclodextrins (CD) are enzymatically modified starches with a wide range of applications in food, pharmaceutical and chemical industries, agriculture and environmental engineering. They are produced from star...
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Article
Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum
Batch cultivations of l-lysine-producing Corynebacterium glutamicum ATCC 21253 were carried out on the different carbon sources, glucose, sucrose and fructose. The time profiles of substrate and product concentra...
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Book
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Article
Quantification of intracellular amino acids in batch cultures of Saccharomyces cerevisiae
The dynamics of intracellular amino acid pools were determined in batch cultures of Saccharomyces cerevisiae. Immediate termination of metabolic activity was found to be necessary for accurate quantification of ...
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Chapter
Integrierte Entwicklung chemischer Prozesse
Sicherheit und Umweltschutz in der chemischen Produktion werden maßgeblich durch die Entwicklung der chemischen Prozesse bestimmt. Dabei gilt es, den Schutz von Mensch und Umwelt zusammen mit Produktqualität u...
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Chapter and Conference Paper
A Microreactor with On-line Mass Spectrometry for the Investigation of Biological Kinetics
A thermostated, stirred reactor of 14 mL volume with integrated pH-probe and control, mass spectrometer membrane probe and dosimeters was constructed. It allowed the measurement of gas exchange rates in anaero...
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Chapter
Carrier Influence for the Treatment of Industrial Wastewaters in Anaerobic Biofilm Fluidized Bed Reactors
The continuous anaerobic degradation of vapour condensate from the cellulose process and brewery wastewater was investigated in parallel experiments using a variety of carriers in eleven single stage biofilm flui...
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Chapter
Comparison of 3 Anaerobic/Aerobic Fluidized Bed Biofilm Systems to Degrade Chlorophenolic Compounds in Pulp Bleaching Effluents
The biodegradation of the 3 chlorophenols, 2, 4, 6-trichlorophenol, 2, 4-dichlorophenol and 4-chlorophenol, contained in real industrial pulp bleaching effluents (chlorination and extraction stages), was studi...
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Chapter
Modelling of Kinetics, Mass Transfer and Mixing Phenomena in 45, 450 and 4500 L Tank Fermenters
The concentration ratio of the excreted products acetoin and butanediol of an oxygen sensitive Bacillus subtilis culture was used to characterize the influence of reactor parameters on oxygen supply. A kinetic mo...
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Chapter
Development of an On-Line Pyrolysis Mass Spectrometry System for the On-Line Analysis of Fermentations
Mass spectrometry has been used already in a number of ways to analyse fermentations:
with a capillary inlet for gas measurement, to monitor the feed and the exhaust gas concen...
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Chapter
Optimisation of a MS-Membrane Probe for the Measurement of Acetoin and Butanediol
Mixing on the macro and also micro-scale and mass transfer may have a profound influence on productivity and yields especially so in large-scale fermentations. Scaling-up of fermentation processes often can on...
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Chapter and Conference Paper
Mass spectrometry for on-line monitoring of biotechnological processes
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Article
The influence of cyclic glucose feeding on a continuous bakers' yeast culture
Except for the pronounced adaptation-hysteresis effect, the pulse experiments exhibited the expected trend: deviation from the steady feed reference curve was greatest at lowest dilution rates. Under condition...
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Article
Experimental Methods for On–Line Mass Spectrometry in Fermentation Technology*
It is shown here how mass spectrometry (MS) can be used for on–line data acquisition in fermentation. MS was applied in this work to analyze gas and liquid phases. Gas phase analysis allows fast and accurate m...
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Article
A kinetic model for growth and synthesis of poly-β-hydroxybutyric acid (PHB) in Alcaligenes eutrophus H 16
A structured model is presented giving a mathematical description of batch cultures of Alcaligenes eutrophus strain H 16 under chemolithoautotrophic growth conditions. A mass-spectrometer with a membrane inlet sy...
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Article
Continuous mass spectrometric measurement of dissolved H2, O2, and CO2 during chemolithoautotrophic growth of Alcaligenes eutrophus strain H 16
Polarographic oxygen electrodes, a mass spectrometer with a membrane inlet system, and a redox electrode were used to measure dissolved H2, O2, and CO2 continuously during chemolithoautotrophic cultivation of Alc...
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Article
Formal kinetics of poly-β-hydroxybutyric acid (PHB) production in Alcaligenes eutrophus H 16 and Mycoplana rubra R 14 with respect to the dissolved oxygen tension in ammonium-limited batch cultures
Under chemolithoautotrophic growth conditions with the organism Alcaligenes eutrophus H16 the exponential growth phase is characterized by two different growth rates, each associated with different specific rates...
