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Protocol
Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications
Biotinylation of proteins is a powerful tool for investigating biological phenomenon, both in vitro and in vivo. Biotinylating reagents that form covalent bonds with several types of amino acid residues are co...
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Chapter
Metabolic Control of Cytochrome P-450cam
Long before cytochrome P-450 was discovered, it was known that the metabolism of steroids was inhibited by CO (Ryan and Engel, 1957). It was a study of carbon monoxide inhibition of steroid metabolism by adren...