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Open AccessMeiosis through three centuries
Meiosis is the specialized cellular program that underlies gamete formation for sexual reproduction. It is therefore not only interesting but also a fundamentally important subject for investigation. An especi...
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Limitations of gamete sequencing for crossover analysis
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Protocol
Diter von Wettstein and The Meiotic Program of Pairing and Recombination
Recombination and pairing are prominent features of meiosis where they play an important role in increasing genetic diversity. In most organisms recombination also plays mechanical roles in mediating pairing o...
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Article
Open AccessThe challenge of evolving stable polyploidy: could an increase in “crossover interference distance” play a central role?
Whole genome duplication is a prominent feature of many highly evolved organisms, especially plants. When duplications occur within species, they yield genomes comprising multiple identical or very similar cop...
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Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
The filamentous fungi Neurospora crassa and Sordaria macrospora are materials of choice for recombination studies because each of the DNA strands involved in meiosis can be visually analyzed using spore-color mut...
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From early homologue recognition to synaptonemal complex formation
This review focuses on various aspects of chromosome homology searching and their relationship to meiotic and vegetative pairing and to the silencing of unpaired copies of genes. Chromosome recognition and pai...
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Indirect intergenic suppression of a radiosensitive mutant of Sordaria macrospora defective in sister-chromatid cohesiveness
Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and there...
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Defective pairing and synaptonemal complex formation in a Sordaria mutant (spo44) with a translocated segment of the nucleolar organizer
The recessive meiotic mutant spo44 of Sordaria macrospora, with 90% ascospore abortion, exhibits striking effects on recombination (67% decrease), irregular segregation of the almost unpaired homologues, and a de...
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Article
One class of mutants with disturbed centromere cleavage and chromosome pairing in Sordaria macrospora
Two recessive mutants spo76 and spo77, altered in U.V. sensitivity, protoplast regeneration and meiotic recombination were isolated in Sordaria macrospora. The suppression of the spo76 phenotype by spo77 suggests...
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Synaptonemal complex and recombination nodules in rye (Secale cereale)
Meiotic prophase in rye was investigated by serial-section reconstruction of pollen mother cell nuclei. In the mid-late zygotene nucleus, all lateral elements were continuous from telomere to telomere, and 9–2...
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Article
Synaptonemal complexes with modified lateral elements in Sordaria humana: development of and relationship to the “recombination nodules”
Meiotic prophase in Sordaria humana has been analyzed by three-dimensional reconstructions of 3 leptotene, 2 zygotene, 10 pachytene and 3 diplotene nuclei. Several notable features emerged. The lateral components...
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Article
Chromosome pairing, recombination nodules and chiasma formation in the basidiomycete coprinus cinereus
Meiosis in the basidiomycete Coprinus cinereus was analyzed by three dimensional reconstructions of nuclei covering the period from leptotene to telophase II. Crosses involving three different strains (JR52, P...
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Synaptonemal complex formation and distribution of recombination nodules in pachytene trivalents of triploid Coprinus cinereus
Synaptonemal complex formation and distribution of recombination nodules were analyzed in trivalents of triploid Coprinus cinereus. The analysis of 14 completely reconstructed nuclei covering the period from e...
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Article
Linkage group-chromosome correlation in Podospora anserina
Two reciprocal translocations have been studied in an attempt to establish a one-to-one assignment of the seven linkage groups of P. anserina to specific chromosomes. Genetical data demonstrated clearly the relat...
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Synchronous sporulation of Saccharomyces cerevisiae at high cell concentrations
A procedure for the sporulation of yeast is described which combines good synchrony and high cell concentration, important features in biochemical studies of meiosis. Meiosis and ascospore formation were follo...
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The pachytene karyotype of Schizophyllum commune analyzed by three dimensional reconstruction of synaptonemal complexes
Three dimensional reconstructions of 15 pachytene nuclei of Schizophyllum commune have revealed in each nucleus 11 distinct synaptonemal complexes, demonstrating a haploid chromosome number of 11. Each bivalen...
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Article
Genes involved in caryogamy and meiosis in Podospora anserina
Ten new mutants affected during caryogamy and first meiotic prophase have been isolated in Podospora anserina. They belong to nine loci, and only one mutant is allelic with a gene previously known. The loci are d...
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Development of the synaptonemal complex and the “recombination nodules” during meiotic prophase in the seven bivalents of the fungus Sordaria macrospora Auersw
Complete reconstruction of seven leptotene, six zygotene, three pachytene and three diplotene nuclei has permitted to follow the pairing process in the Ascomycete Sordaria macrospora. The seven bivalents in Sorda...
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Article
The synaptonemal complex and the spindle plaque during meiosis in yeast
Meiosis in Saccharomyces cerevisiae proceeds principally in the same manner as in other Ascomycetes. Leptotene is characterized by unpaired lateral components and pachytene by the presence of extensive synaptonem...
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Article
Division spindle and centrosomal plaques during mitosis and meiosis in some Ascomycetes
The behaviour of the division spindle and centrosomal plaques is described in four species of Ascomycetes (Ascobolus immersus, Ascobolus stercorarius, Podospora anserina and Podospora setosa) studied by light and...