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Open AccessA universal deep-learning model for zinc finger design enables transcription factor reprogramming
Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the genome provide an effective strategy for programmable regulation of gene expression, with many potential therapeutic applicati...
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Open AccessEngineered dual selection for directed evolution of SpCas9 PAM specificity
The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interacti...
