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  1. Article

    Open Access

    A universal deep-learning model for zinc finger design enables transcription factor reprogramming

    Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the genome provide an effective strategy for programmable regulation of gene expression, with many potential therapeutic applicati...

    David M. Ichikawa, Osama Abdin, Nader Alerasool… in Nature Biotechnology (2023)

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  2. Article

    Open Access

    Engineered dual selection for directed evolution of SpCas9 PAM specificity

    The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interacti...

    Gregory W. Goldberg, Jeffrey M. Spencer, David O. Giganti… in Nature Communications (2021)

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  3. No Access

    Article

    Differentiation of human and murine induced pluripotent stem cells to microglia-like cells

    Pandya et al. describe a protocol to differentiate human and mouse iPSCs into cells with the phenotype, transcriptional profile and functional properties of microglia. The treatment of murine intracranial maligna...

    Hetal Pandya, Michael J Shen, David M Ichikawa, Andrea B Sedlock… in Nature Neuroscience (2017)