7 Result(s)
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Article
The furin cleavage site in the SARS-CoV-2 spike protein is required for transmission in ferrets
SARS-CoV-2 entry requires sequential cleavage of the spike glycoprotein at the S1/S2 and the S2ʹ cleavage sites to mediate membrane fusion. SARS-CoV-2 has a polybasic insertion (PRRAR) at the S1/S2 cleavage si...
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Article
Open AccessDeep splicing plasticity of the human adenovirus type 5 transcriptome drives virus evolution
Viral genomes have high gene densities and complex transcription strategies rendering transcriptome analysis through short-read RNA-seq approaches problematic. Adenovirus transcription and splicing is especial...
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Article
Open AccessProteomics informed by transcriptomics for characterising differential cellular susceptibility to Nelson Bay orthoreovirus infection
Nelson Bay orthoreovirus (NBV) is a fusogenic bat borne virus with an unknown zoonotic potential. Previous studies have shown that NBV can infect and replicate in a wide variety of cell types derived from thei...
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Open AccessProteomics informed by transcriptomics for characterising active transposable elements and genome annotation in Aedes aegypti
Aedes aegypti is a vector for the (re-)emerging human pathogens dengue, chikungunya, yellow fever and Zika viruses. Almost half of the Ae. aegypti genome is comprised of transposable e...
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De novo derivation of proteomes from transcriptomes for transcript and protein identification
Integration of transcriptomic and proteomic data allows more detailed annotation of proteomes of model and non-model species.
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Article
A plasmid-encoded dihydrofolate reductase from trimethoprim-resistant bacteria has a novel D2-symmetric active site
Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR) exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is a 34,000 Mr homotetramer which exists in equilibrium wit...
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Article
Structure of and kinetic channelling in bifunctional dihydrofolate reductase–thymidylate synthase
The bifunctional enzyme dihydrofolate reductase–thymidylate synthase catalyses both the reductive methylation of 2′–deoxyuridylate and the subsequent reduction of dihydrofolate to yield 2′–deoxythymidylate and...
