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Article
Open AccessTumor cell integrin β4 and tumor stroma E-/P-selectin cooperatively regulate tumor growth in vivo
The immunological composition of the tumor microenvironment has a decisive influence on the biological course of cancer and is therefore of profound clinical relevance. In this study, we analyzed the cooperati...
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Article
Open AccessFra-2 overexpression upregulates pro-metastatic cell-adhesion molecules, promotes pulmonary metastasis, and reduces survival in a spontaneous xenograft model of human breast cancer
The transcription factor Fra-2 affects the invasive potential of breast cancer cells by dysregulating adhesion molecules in vitro. Previous results suggested that it upregulates the expression of E- and P-sele...
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Article
Open AccessIntegrin alpha-V is an important driver in pancreatic adenocarcinoma progression
Mesothelial E- and P-selectins substantially mediate the intraperitoneal spread of Pancreatic ductal adenocarcinoma (PDA) cells in xenograft models. In the absence of selectins in the host, the integrin subuni...
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Article
Open AccessCEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells
We investigated the functional role of CEACAM1 in a spontaneous metastasis xenograft model of human melanoma in scid mice using BRAF wildtype MeWo cells with and without RNAi mediated knockdown of CEACAM1. Tum...
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Article
Cell adhesion molecules in metastatic neuroblastoma models
Several cell adhesion molecules (CAMs) including selectins, integrins, cadherins and immunoglobulin-like CAMs are involved in leukocyte adhesion especially at sites of inflammation. In cancer cells, these CAMs...
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Article
Influence of L1-CAM expression of breast cancer cells on adhesion to endothelial cells
Expression of the adhesion molecule L1-CAM (L1) has been shown to correlate with early recurrence in breast cancer. Here, we investigated whether L1-CAM expression of breast cancer cells might influence adhere...
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Article
The interaction between CD44 on tumour cells and hyaluronan under physiologic flow conditions: implications for metastasis formation
The adhesion of tumour cells to the endothelial cells of blood vessels of the microcirculation represents a crucial step in haematogenous metastasis formation. Similar to leukocyte extravasation, selectins med...
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Protocol
RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example
Specific gene silencing using small hairpin RNA (shRNA) constructs offer researchers the possibility to study the influence of a single protein in the metastatic process. The role of the cellular adhesion mole...
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Article
Adhesion of small cell lung cancer cells to E- and P-Selectin under physiological flow conditions: implications for metastasis formation
Haematogenous metastasis of small cell lung cancer (SCLC) is still a poorly understood process and represents the life threatening event in this malignancy. In particular, the rate-limiting step within the met...
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Article
Open AccessEstablishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung
Pancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As...
